Patents by Inventor Sergey Vasilievich Smirnov

Sergey Vasilievich Smirnov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11060121
    Abstract: The present invention provides a method for producing glycine or a salt thereof by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity. The bacterium can be, for example, a coryneform bacterium or a bacterium belonging to the family Enterobacteriaceae.
    Type: Grant
    Filed: June 23, 2020
    Date of Patent: July 13, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova
  • Publication number: 20210115484
    Abstract: A method for enzymatic sulfurylation of a substrate is provided which includes the steps of reacting the substrate with 3?-phosphoadenosine-5?-phosphosulfate (PAPS) in a medium containing a bacterium belonging to the family Enterobacteriaceae to produce a sulfated derivative of the substrate, and collecting the sulfated derivative from the medium, wherein the bacterium has been modified to produce, at least, a protein having sulfotransferase activity, and to attenuate expression of an aphA gene, a cysQ gene, or a cpdB gene, or a combination of these.
    Type: Application
    Filed: December 23, 2020
    Publication date: April 22, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Irina Lvovna Tokmakova
  • Publication number: 20200325507
    Abstract: The present invention provides a method for producing glycine or a salt thereof by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity. The bacterium can be, for example, a coryneform bacterium or a bacterium belonging to the family Enterobacteriaceae.
    Type: Application
    Filed: June 23, 2020
    Publication date: October 15, 2020
    Applicant: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova
  • Patent number: 10077447
    Abstract: The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.
    Type: Grant
    Filed: October 27, 2015
    Date of Patent: September 18, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova, Hidemi Fujii, Masakazu Sugiyama
  • Patent number: 9428783
    Abstract: The present invention describes novel bacterial L-amino acids ?-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids ?-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids ?-ligases, is described.
    Type: Grant
    Filed: January 7, 2015
    Date of Patent: August 30, 2016
    Assignee: Ajinomoto Co., Inc.
    Inventors: Sergey Vasilievich Smirnov, Pavel Mikhailovich Sokolov, Takayuki Ito
  • Publication number: 20160060640
    Abstract: The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.
    Type: Application
    Filed: October 27, 2015
    Publication date: March 3, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova, Hidemi Fujii, Masakazu Sugiyama
  • Publication number: 20150197782
    Abstract: The present invention describes novel bacterial L-amino acids ?-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids ?-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids ?-ligases, is described.
    Type: Application
    Filed: January 7, 2015
    Publication date: July 16, 2015
    Applicant: Ajinomoto Co., Inc.
    Inventors: Sergey Vasilievich SMIRNOV, Pavel Mikhailovich Sokolov, Takayuki Ito
  • Patent number: 9051591
    Abstract: The presently disclosed subject matter provides a bacterium of Enterobacteriaceae family producing L-aspartic acid or an L-aspartic acid-derived metabolite modified to have aspartate dehydrogenase and a method for producing L-aspartic acid or an L-aspartic acid-derived metabolite, such as L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine, using such bacterium.
    Type: Grant
    Filed: July 6, 2012
    Date of Patent: June 9, 2015
    Assignee: AJINOMOTO CO., INC.
    Inventors: Tatyana Mikhailovna Kuvaeva, Sergey Vasilievich Smirnov, Olga Nikolaevna Ivanova, Aleksandr Dmitrievich Kivero, Joanna Yosifovna Katashkina
  • Patent number: 8524476
    Abstract: A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: September 3, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko, Tatyana Aleksandrovna Bachina, Yuki Imabayashi, Masakazu Sugiyama, Shunichi Suzuki
  • Patent number: 8367381
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: February 5, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Patent number: 8367382
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: February 5, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Publication number: 20120329105
    Abstract: The presently disclosed subject matter provides a bacterium of Enterobacteriaceae family producing L-aspartic acid or an L-aspartic acid-derived metabolite modified to have aspartate dehydrogenase and a method for producing L-aspartic acid or an L-aspartic acid-derived metabolite, such as L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine, using such bacterium.
    Type: Application
    Filed: July 6, 2012
    Publication date: December 27, 2012
    Inventors: Tatyana Mikhailovna Kuvaeva, Sergey Vasilievich Smirnov, Olga Nikolaevna Ivanova, Aleksandr Dmitrievich Kivero, Joanna Yosifovna Katashkina
  • Patent number: 8273562
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: September 25, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20120115203
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 10, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Publication number: 20120107884
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 3, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20120107883
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 3, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Patent number: 8114651
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: March 27, 2009
    Date of Patent: February 14, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20100330622
    Abstract: A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
    Type: Application
    Filed: June 21, 2010
    Publication date: December 30, 2010
    Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko, Tatyana Aleksandrovna Bachina, Yuki Imabayashi, Masakazu Sugiyama, Shunichi Suzuki
  • Publication number: 20100323409
    Abstract: A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using an L-isoleucine-producing bacterium transformed with a DNA fragment containing a gene coding for a protein having L-isoleucine dioxygenase activity; and having the ability to produce (2S,3R,4S)-4-hydroxy-L-isoleucine.
    Type: Application
    Filed: June 21, 2010
    Publication date: December 23, 2010
    Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko
  • Patent number: 7670822
    Abstract: A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and ?-ketobutyric acid using a novel aldolase, which is derived from the genus Arthrobacter.
    Type: Grant
    Filed: September 18, 2008
    Date of Patent: March 2, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Tomohiro Kodera, Kazuhiko Matsui