Abstract: Provided herein are improved methods of manufacturing cells, including T cells and CAR T cells. Also provided herein are methods of manufacturing cells, such as T cells and CAR T cells, obtainable from blood using a method incorporating membrane filtration and Ammonium-Chloride-Potassium (ACK) buffer to isolate the cells from other blood components.

Abstract: A communication link is established between a first wireless device and a communication network using a network communication protocol when it is determined at the first wireless device that a communication link with an access node is not detected. The first wireless device transmits an indication that the first wireless device can provide wireless communications to a second wireless device using a cellular radio access technology. In response to a communication channel request from the second wireless device, the first wireless device grants a communication channel to the second wireless device, and the second wireless device is assigned a network address. A request is received at the first wireless device to establish a communication session for the second wireless device over the communication network, and the communication session is established by the wireless device for the second wireless device over the communication network.

Abstract: A metering device for aspirating and dispensing a liquid includes a housing; a pumping medium, preferably a gas containing chamber contained within the housing; a channel having a proximate end in fluid communication with the chamber and a distal end in fluid communication with an external environment; a heat or cold source providing a source of heat or cold to the gas containing chamber; and a temperature sensor for measuring the temperature inside the chamber. In a preferred embodiment, the device further includes a pressure sensor for measuring gas pressure inside the chamber, or multiple chambers.

Abstract: The invention is an impulse wrench that employs a fluid coupling between its anvil and hammer members. The tool includes a pulse cylinder that forms the tool's hammer and has a shaped inner surface that defines a side wall of a fluid-filled chamber. An end portion of the anvil is received within the chamber and includes two retractable vanes that sweep the pulse cylinder's inner surface when the pulse cylinder is rotating about the anvil. To achieve only a single impact per revolution of the pulse cylinder, fluid bypass channels are employed to intermittently allow fluid to pass around the vanes. In addition, the tool includes a unique torque-sensing shut-off mechanism that is engaged to the tool's motor and makes use of inertia force to actuate a power shut-off device.