Abstract: The invention is directed to quantitative lateral flow assay strips for a quantitative analysis of specific analyte by a point of care scanner. The quantitative lateral flow assay strip includes a body portion dimensioned for operable interface with the optical reader portion of the point of care scanner. The strip further including a sample pad for deposit of a known quantity of a biological sample containing an unknown quantity of the specific analyte and a conjugate pad located adjacent to the sample pad. The conjugate pad having an analyte/antibody conjugated to an optically detectable particle, which are all fixed to the conjugate pad in a dry state. The strip also has a capillary flow portion adjacent to the conjugate pad having a pore size selected to facilitate sample flow through the body portion and a test line portion having an anti-analyte antibody fixed in a dry state to the body portion of the strip. The strip may also include a control line having anti-conjugate antibody fixed in a dry state.