Abstract: A method to achieve controlled conductivity in microfluidic devices, and a device formed thereby. The method comprises forming a microchannel or a well in an insulating material, and ion implanting at least one region of the insulating material at or adjacent the microchannel or well to increase conductivity of the region.

Abstract: Disclosed herein are micropurification cards, systems, systems and methods, backspace. A micropurification cards include a plurality of fluidic components capable of extracting molecules from samples. Samples include biological cells and the extracted molecules include nucleic acids.

Abstract: A microfluidic system and method for employing it to control fluid temperatures of fluids residing within microchannels of a microfluidic device. The microfluidic device is provided with a top layer and a bottom layer and microchannels configured therebetween. Temperature of the fluid within the microchannels is controlled in various ways including the use of electrical resistive heating elements and by providing zones located in contact with the top and bottom layers of the microfluidic device for circulating heat transfer of fluid therein.

Abstract: A method to achieve controlled conductivity in microfluidic devices, and a device formed thereby. The method comprises forming a microchannel or a well in an insulating material, and ion implanting at least one region of the insulating material at or adjacent the microchannel or well to increase conductivity of the region.

Abstract: In a method for performing a hot start polymerase chain reaction, a reaction mixture including a primer, a template molecule, and a buffer—but no polymerase enzyme—is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A polymerase enzyme is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the hot start polymerase chain reaction is performed. In a method for performing a temperature mediated reaction, a first component of the temperature mediated reaction is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A second component of the temperature mediated reaction is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the temperature mediated reaction is performed.

Abstract: Microfluidic devices with improved channel and reservoir configurations are provided. More specifically, efficient microfluidic devices for the performance of temperature mediated reactions are provided. These reactions can be performed in an ease of use and efficient manner so as to enable high through put of multiple samples. Methods for performing temperature mediated reactions using the microfluidic devices with improved channel and reservoir configurations have also been provided.

Abstract: Microfluidic devices with improved channel and reservoir configurations are provided. More specifically, efficient microfluidic devices for the performance of temperature mediated reactions are provided. These reactions can be performed in an ease of use and efficient manner so as to enable high through put of multiple samples. Methods for performing temperature mediated reactions using the microfluidic devices with improved channel and reservoir configurations have also been provided.

Abstract: Microfluidic devices, systems and methods that are operated and/or configured to enhance the passage of electrical current from power supplies to the fluids within the system. The devices, methods and systems utilize alternate electrode and system configurations to permit transfer of current at lower voltages, minimize water hydrolysis at the electrode/fluid interface and/or permit accurate in situ measurement of the parameters of processes carried out in those devices and systems.

Abstract: Microfluidic devices with improved channel and reservoir configurations are provided. More specifically, efficient microfluidic devices for the performance of temperature mediated reactions are provided. These reactions can be performed in an ease of use and efficient manner so as to enable high through put of multiple samples. Methods for performing temperature mediated reactions using the microfluidic devices with improved channel and reservoir configurations have also been provided.