Patents by Inventor Seth Shipman

Seth Shipman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Modified mitochondrion and methods of use thereof
    Patent number: 12460230
    Abstract: Methods and compositions for introducing an exogenous mitochondrion harvested from a donor cell into a recipient cell are provided. Also provided are methods and compositions for producing a modified mitochondrion and treating mitochondrial disease in an individual with the mitochondrial disease. Further provided are modified mitochondria, genetically modified mitochondria, genetically modified cells, isolated compositions and pharmaceutical compositions for practicing the subject methods.
    Type: Grant
    Filed: February 10, 2020
    Date of Patent: November 4, 2025
    Assignee: The J. David Gladstone Institutes
    Inventor: Seth Shipman
  • Dual Cut Retron Editors for Genomic Insertions and Deletions
    Publication number: 20250243516
    Abstract: Described herein are retron-related constructs, expression systems, and methods for precisely deleting, inserting and/or replacing genomic DNA within cells. The inventors have found that CRISPR “single-cutter” methods are substantially less efficient at insertion/replacement of large fragments in comparison to the dual-cutter constructs, expression systems, and methods described herein.
    Type: Application
    Filed: August 24, 2023
    Publication date: July 31, 2025
    Inventors: Seth Shipman, Santiago C. Lopez
  • Continuous Multiplexed Phage Genome Engineering Using a Retron Editing Template
    Publication number: 20250230429
    Abstract: Systems and methods for editing bacteriophages are described herein.
    Type: Application
    Filed: March 9, 2023
    Publication date: July 17, 2025
    Inventors: Seth Shipman, Chloe Fishman, Santi Bhattarai-Kline
  • METHODS OF ASSESSING ENGINEERED RETRON ACTIVITY, AND USES THEREOF
    Publication number: 20250223586
    Abstract: Methods and compositions are described herein that are useful for analyzing and identifying retron systems with improved replication fidelity and efficiency. The methods and compositions described herein facilitate the engineering, quantification, and identification of retron elements for precise genome engineering.
    Type: Application
    Filed: March 24, 2023
    Publication date: July 10, 2025
    Inventor: Seth Shipman
  • RT-DNA Fidelity and Retron Genome Editing
    Publication number: 20250215422
    Abstract: Described herein are compositions and methods that can analyze optimal systems for improved fidelity and frequency of genomic editing. The compositions and methods involve use of modified retrons.
    Type: Application
    Filed: March 24, 2023
    Publication date: July 3, 2025
    Inventor: Seth Shipman
  • Production of reverse transcribed DNA (RT-DNA) using a retron reverse transcriptase from exogenous RNA
    Publication number: 20250215458
    Abstract: Described herein are methods and compositions for transfecting retron RNAs. In vitro transcription can be used to synthesize the retron RNA. The methods and compositions facilitate precise engineering of retron RNAs and can readily be adapted to generate an abundance of different donor DNAs and guide RNAs within cells.
    Type: Application
    Filed: March 24, 2023
    Publication date: July 3, 2025
    Inventors: Seth Shipman, Sierra Lear
  • MODIFIED BACTERIAL RETROELEMENT WITH ENHANCED DNA PRODUCTION
    Publication number: 20250188442
    Abstract: Engineered retrons, modified to enhance production of multicopy single-stranded DNA (msDNA), are provided. In addition, vector systems encoding such engineered retrons and methods of using engineered retrons and vector systems encoding them in various applications such as CRISPR/Cas-mediated genome editing, recombineering, cellular barcoding, and molecular recording are also disclosed.
    Type: Application
    Filed: November 15, 2024
    Publication date: June 12, 2025
    Inventor: Seth Shipman
  • Precise Genome Editing Using Retrons
    Publication number: 20250043269
    Abstract: Described herein are compositions and methods that provide precise editing of cells, including mammalian cells and human cells. The compositions and methods utilize retrons as repair donors, retron reverse transcriptases to make those retron repair donors, retron-encoded guide RNAs, and CRISPR nucleases.
    Type: Application
    Filed: November 3, 2022
    Publication date: February 6, 2025
    Inventors: Santiago C. Lopez, Seth Shipman
  • Retroelement-generated transcription factor decoys
    Publication number: 20250011787
    Abstract: Described herein are retrons that are engineered to have binding sites for transcription factors, as well as compositions, systems, and methods for obtaining and using such engineered retrons.
    Type: Application
    Filed: October 21, 2022
    Publication date: January 9, 2025
    Inventors: Seth Shipman, Katherine Crawford
  • MODIFIED BACTERIAL RETROELEMENT WITH ENHANCED DNA PRODUCTION
    Publication number: 20230125704
    Abstract: Engineered retrons, modified to enhance production of multicopy single-stranded DNA (msDNA), are provided. In addition, vector systems encoding such engineered retrons and methods of using engineered retrons and vector systems encoding them in various applications such as CRISPR/Cas-mediated genome editing, recombineering, cellular barcoding, and molecular recording are also disclosed.
    Type: Application
    Filed: December 1, 2022
    Publication date: April 27, 2023
    Inventor: Seth Shipman
  • MODIFIED BACTERIAL RETROELEMENT WITH ENHANCED DNA PRODUCTION
    Publication number: 20220307007
    Abstract: Engineered retrons, modified to enhance production of multicopy single-stranded DNA (msDNA), are provided. In addition, vector systems encoding such engineered retrons and methods of using engineered retrons and vector systems encoding them in various applications such as CRISPR/Cas-mediated genome editing, recombineering, cellular barcoding, and molecular recording are also disclosed.
    Type: Application
    Filed: September 11, 2020
    Publication date: September 29, 2022
    Inventor: Seth Shipman
  • MODIFIED MITOCHONDRION AND METHODS OF USE THEREOF
    Publication number: 20220145330
    Abstract: Methods and compositions for introducing an exogenous mitochondrion harvested from a donor cell into a recipient cell are provided. Also provided are methods and compositions for producing a modified mitochondrion and treating mitochondrial disease in an individual with the mitochondrial disease. Further provided are modified mitochondria, genetically modified mitochondria, genetically modified cells, isolated compositions and pharmaceutical compositions for practicing the subject methods.
    Type: Application
    Filed: February 10, 2020
    Publication date: May 12, 2022
    Inventor: Seth Shipman