Abstract: Provided is a method for evaluating immunotoxicity of a chemical substance to a mammal, including step 1: bringing a chemical substance into contact with a mammalian cell for immunotoxicity evaluation, the mammalian cell having a first reporter gene under control of a constant expression promoter and a second reporter gene under control of a promoter for immunotoxicity evaluation incorporated in a transiently or stably expressible manner; and step 2: evaluating an increase or decrease in expression of the second reporter gene relative to that of the first reporter gene and an increase or decrease in expression of the first reporter gene in the presence and absence of the chemical substance.

Abstract: Provided is an objective and convenient index for judging full recovery from atopic dermatitis and severity thereof. The present invention provides a diagnosis assisting method for determining a treatment policy for an atopic dermatitis subject under the treatment, said method comprising: measuring the expression value of SCCA-1 in skin horny cells of the subject; i) when the SCCA-1 expression value is not statistically significantly higher than the level of those who do not suffer from atopic dermatitis, then determining that the continuation of the treatment for the disease becomes unnecessary thanks to the treatment; and, in the case where the continuation of the treatment is determined as still necessary, ii) ranking the severity of atopic dermatitis of the subject using the SCCA-1 expression value as an index and determining an adequate treatment method for atopic dermatitis to be given to the subject depending on the rank.