Abstract: The present invention relates to a vacuum drying method for botulinum toxin, and specifically to a vacuum drying method for botulinum toxin, comprising the steps of: a) first drying of a solution containing botulinum toxin at a temperature of 5° C. to 30° C. under a pressure of 0.01 to 2 mmHg; b) a second drying of the first-dried product obtained in step a) at a temperature of 5° C. to 20° C. under a pressure of 0.01 to 1 mmHg; and c) final drying of the second-dried product obtained in step b) at a temperature of 5° C. to 30° C. under a pressure of 0.01 to 0.5 mmHg. The present invention can advantageously improve yield, stability and manufacturing efficiency, compared to conventional methods for vacuum drying botulinum toxin by freeze-drying.