Abstract: An anode for a secondary battery is provided, the anode for a secondary battery including: an anode current collector; a first anode mixture layer formed on at least one surface of the anode current collector, and including a first silicon-based active material and a first conductive material; and a second anode mixture layer formed on the first anode mixture layer, and including a second silicon-based active material and a second conductive material, wherein a content of the first silicon-based active material in the first anode mixture layer is lower than a content of the second silicon-based active material in the second anode mixture layer, and a Radial Breathing Mode (RBM) peak is observed in a Raman spectrum obtained from a surface of the second anode mixture layer.

Abstract: A glass article used as a cover glass of a flexible display device has values of indices consistent with results of a pen drop test and excellent impact resistance characteristics.

Abstract: Disclosed is a method for producing soluble CRM197 protein at high yield by overexpressing the protein in the cytoplasm of E. coli. The method includes a step of culturing recombinant E. coli harboring an expression plasmid carrying a gene sequence coding for a recombinant CRM197 protein under a condition suitable for expression of the recombinant CRM197 protein which has a histidine-tag attached to the C-terminus of CRM197 and a maltose-binding protein (MBP) attached to the N-terminus of CRM197. The method further includes a step of purifying after the culturing, wherein the step of purifying comprises a step of treating with tobacco etch virus (TEV) protease to remove the maltose-binding protein and a step of removing the histidine-tag via histidine-tag affinity chromatography.

Abstract: Disclosed is a method for expressing and purifying insoluble proteins of CRM197. The method includes: culturing a transformant transformed with an expression vector containing a gene encoding CRM197 protein; obtaining a culture of the transformant and disrupting the cell to recover an inclusion body; solubilizing the inclusion body; treating the solubilized inclusion body with a refolding reaction mixture and performing a refolding process; and purifying the refolded CRM197 protein by chromatography.

Abstract: Disclosed is a method for producing soluble CRM197 protein at high yield by overexpressing the protein in the cytoplasm of E. coli. The method includes a step of culturing recombinant E. coli harboring an expression plasmid carrying a gene sequence coding for a recombinant CRM197 protein under a condition suitable for expression of the recombinant CRM197 protein which has a histidine-tag attached to the C-terminus of CRM197 and a maltose-binding protein (MBP) attached to the N-terminus of CRM197. The method further includes a step of purifying after the culturing, wherein the step of purifying comprises a step of treating with tobacco etch virus (TEV) protease to remove the maltose-binding protein and a step of removing the histidine-tag via histidine-tag affinity chromatography.

Abstract: There is provided with a complex ocean power system combining ocean current power generation for generating electricity by forming a plurality of ocean current generators in front and rear of sluice structures of tidal power dams and sluice power generation for generating electricity by forming a plurality of ocean current generators in sluice conduits of the sluice structures, comprising: constructing barrages, which cross over the sea, to make up a lake; installing sluice structures of tidal power dams between the barrages 10 to generate electricity by changing a potential energy difference between seawaters caused by tides and ebbs to kinetic energy; installing ocean current generators in front and rear of the sluice structures and in the sluice conduits of the sluice structures to generate electricity by rotating turbine blades using the flow of the incoming seawater from a sea side into a lake side during flood tide and the flow of the seawater discharged from the lake side into the sea side during ebb t

Abstract: An integrated power system combining tidal power generation and ocean current power generation comprises: constructing barrages across the sea to make up a lake; installing turbine structures of a tidal power plant and sluice structures of a tidal power dam for generating electricity by using the potential energy difference between seawaters caused by tides and ebbs; forming an ocean current power park in a lake side by installing a plurality of ocean current generators, for generating electricity by using the flow of the seawater discharged through turbine generators, in a rear lake side of the turbine structures of the tidal power plant; and forming an ocean current power park in a sea side by installing ocean current generators, for generating electricity by using the seawater with the fast speed discharged into the sea through sluice gates, in a rear sea side of the sluice structures of the tidal power dam.