Abstract: An imprinting photomask including: a transparent substrate; a light blocking pattern provided on the transparent substrate; and a dry film resist (DFR) pattern provided on the light blocking pattern.

Abstract: In one example, a semiconductor device can comprise a substrate, a device stack, first and second internal interconnects, and an encapsulant. The substrate can comprise a first and second substrate sides opposite each other, a substrate outer sidewall between the first substrate side and the second substrate side, and a substrate inner sidewall defining a cavity between the first substrate side and the second substrate side. The device stack can be in the cavity and can comprise a first electronic device, and a second electronic device stacked on the first electronic device. The first internal interconnect can be coupled to the substrate and the device stack. The encapsulant can cover the substrate inner sidewall and the device stack and can fill the cavity. Other examples and related methods are disclosed herein.

Abstract: Disclosed are a vector comprising a first expression cassette comprising a DmpR ribosomal binding site as a transcriptional regulator and a promoter operably linked thereto; and a second expression cassette comprising a sequence encoding a target polypeptide, a ribosomal binding site, and a Po promoter operably linked thereto, and a methanotroph transformed by the vector.

Abstract: Various embodiments of the present invention relate to a method for detecting and controlling a battery status by using a sensor, and an electronic device using the same, the electronic device comprising: a housing; an accommodation part arranged inside the housing and including at least one gas sensor; a battery arranged inside the housing; a memory for storing information, acquired by the at least one gas sensor, on gas leaked from the battery and operation control information of the electronic device; and a processor electrically connected to the memory, wherein the processor is configured to acquire a detection signal of gas leaked from the battery by using the at least one gas sensor, and control the operation of at least a part of the electronic device and/or charging characteristics of the battery when the acquired gas detection signal exceeds a predetermined threshold value, so as to detect, in real time, a gas leakage of the battery occurring because of the exposure of the electronic device to high t

Abstract: An electronic device is provided. The electronic device includes a display disposed toward a first surface, a housing including an opening formed toward a second surface, a tray including a hole formed toward the second surface, a sensor module that is able to be seated on the tray, a connector disposed adjacent to the opening inside the electronic device to receive the tray and the sensor module, and a processor configured to control the sensor module when the sensor module is inserted into the connector. The sensor module includes a gas sensor, a sensor chamber, and a pipe forming a path allowing gas, which is introduced through the hole, to move into the sensor chamber.

Abstract: An electronic device is provided. The electronic device includes a display disposed toward a first surface, a housing including an opening formed toward a second surface, a tray including a hole formed toward the second surface, a sensor module that is able to be seated on the tray, a connector disposed adjacent to the opening inside the electronic device to receive the tray and the sensor module, and a processor configured to control the sensor module when the sensor module is inserted into the connector. The sensor module includes a gas sensor, a sensor chamber, and a pipe forming a path allowing gas, which is introduced through the hole, to move into the sensor chamber.

Abstract: The present invention relates to a method for analyzing the interaction between a binding protein and a target material, including measuring the interaction between the binding protein and a target material using an interaction trapper (IT) cell. The IT cell has the binding protein displayed on the surface of intracellular inclusion bodies, i.e., insoluble aggregates, by expressing a fusion protein which forms active protein particles containing the binding protein. The method includes increasing cell permeability without affecting the activity of the binding protein displayed on the cells and genetic information.

Abstract: Various embodiments of the present invention relate to a method for detecting and controlling a battery status by using a sensor, and an electronic device using the same, the electronic device comprising: a housing; an accommodation part arranged inside the housing and including at least one gas sensor; a battery arranged inside the housing; a memory for storing information, acquired by the at least one gas sensor, on gas leaked from the battery and operation control information of the electronic device; and a processor electrically connected to the memory, wherein the processor is configured to acquire a detection signal of gas leaked from the battery by using the at least one gas sensor, and control the operation of at least a part of the electronic device and/or charging characteristics of the battery when the acquired gas detection signal exceeds a predetermined threshold value, so as to detect, in real time, a gas leakage of the battery occurring because of the exposure of the electronic device to high t

Abstract: Disclosed is a gas sensor module for an electronic device, the gas sensor module comprising: a gas sensor mounted on a circuit board and identifying gas introduced into a sensor unit; a sensor frame formed in a box shape with one open surface and having a hole communicating with the outside, the sensor frame covering the gas sensor to isolate the gas sensor from the outside; and a connection unit formed at an edge of the open surface of the sensor frame and coupled to the circuit board. Other embodiments are possible.

Abstract: An electronic device is provided. The electronic device includes a display disposed toward a first surface, a housing including an opening formed toward a second surface, a tray including a hole formed toward the second surface, a sensor module that is able to be seated on the tray, a connector disposed adjacent to the opening inside the electronic device to receive the tray and the sensor module, and a processor configured to control the sensor module when the sensor module is inserted into the connector. The sensor module includes a gas sensor, a sensor chamber, and a pipe forming a path allowing gas, which is introduced through the hole, to move into the sensor chamber.

Abstract: The present application relates to a method for detecting ligand using a biosensor applied the FRET (fluorescence resonance energy transfer) phenomenon. More particularly, the method may be used for simply detecting a ligand in a sample by measuring the FRET of a biosensor under the conditions in which a specific critical temperature is maintained. The method may use a phenomenon in which a ligand-binding protein in a biosensor shows reversible unfolding at a temperature higher than the specific critical temperature and the level of the unfolding changes depending on the concentration of a ligand. The method can be widely applied to a variety of kinds of FRET biosensors using the ligand-binding protein.

Abstract: A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification.

Abstract: The present invention relates to a method for analyzing the interaction between a binding protein and a target material, including measuring the interaction between the binding protein and a target material using an interaction trapper (IT) cell. The IT cell has the binding protein displayed on the surface of intracellular inclusion bodies, i.e., insoluble aggregates, by expressing a fusion protein which forms active protein particles containing the binding protein. The method includes increasing cell permeability without affecting the activity of the binding protein displayed on the cells and genetic information.

Abstract: A method of performing high-throughput screening of various enzymatic activities with high sensitivity using artificial genetic circuits is provided. Particularly, the invention is screening and quantifying the activity of isoprene biosynthesis enzymes using an artificial genetic circuit capable of sensing isoprene. The artificial genetic circuit comprises an isoprene-sensing transcriptional regulator which recognizes isoprene, at least one reporter gene, a isoprene-sensing transcriptional regulator binding region and promoters for genes encoding isoprene-sensing transcriptional regulators and reporter proteins. The artificial genetic circuit detects isoprene liberated from many enzymatic reactions and measures the activity of reporter genes. This system is widely applicable for high throughput and quantitative screening of isoprene biosynthesis enzymes and MEP/MVA pathway enzymes. Therefore, the invention can be advantageously used in the protein engineering technology for enzyme modification.

Abstract: The present invention relates to methods for dynamically detecting the interactions between various materials including bioactive molecules and for detecting target molecules. More specifically, the present invention relates to a method for dynamically detecting bait-prey interactions and a method for easily detecting target molecules which blocks or activates the interactions, the method comprising: allowing a material capable of forming a nano-assembly matrix, a bait and a prey to interact with each other, and analyzing whether a nano-assembly matrix is formed by the interaction between the bait and the prey in vitro or in vivo; or allowing a material capable of forming a nano-assembly matrix, a bait and a prey to interact with each other, inducing the formation of a nano-assembly matrix by a mediator (regulator) material in vitro or in vivo, and then analyzing whether the prey and the bait co-localizes on the nano-assembly matrix.

Abstract: The present invention provides for a biochemically and physically defined extracellular microenvironment prepared from mussel adhesive proteins recombinantly functionalized with a variety of bioactive peptides such as extracellular matrix-derived or growth factor-derived peptides. The synthetic extracellular microenvironment can be customized to regulate cellular behavior such as cell adhesion, growth, differentiation and morphogenesis in a variety of cells. The invention provides for a modulatory extracellular microenvironment by presenting a matricryptic site into said mussel adhesive proteins. The invention also provides for devices and methods for screening for optimal combinations of ECM derived peptide motifs in order to create a microenvironment that can regulate specific cellular behavior.

Abstract: The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.

Abstract: A method of performing high-throughput screening of various enzymatic activities with high sensitivity using artificial genetic circuits is provided. Particularly, the invention is screening and quantifying the activity of isoprene biosynthesis enzymes using an artificial genetic circuit capable of sensing isoprene. The artificial genetic circuit comprises an isoprene-sensing transcriptional regulator which recognizes isoprene, at least one reporter gene, a isoprene-sensing transcriptional regulator binding region and promoters for genes encoding isoprene-sensing transcriptional regulators and reporter proteins. The artificial genetic circuit detects isoprene liberated from many enzymatic reactions and measures the activity of reporter genes. This system is widely applicable for high throughput and quantitative screening of isoprene biosynthesis enzymes and MEP/MVA pathway enzymes. Therefore, the invention can be advantageously used in the protein engineering technology for enzyme modification.

Abstract: The present invention relates to a metagenome-derived alkaline phosphatase, and more particularly to a novel, metagenome-derived alkaline phosphatase screened using a artificial genetic circuit that detects phenolic compounds, and a preparation method thereof. A novel alkaline phosphatase according to the present invention has high activity of dephosphorylating DNA and can be inactivated rapidly by simple heat treatment. Thus, it can be used for a dephosphorylation reaction so that genetic manipulations, including genetic cloning, become efficient. In addition, it can be actively expressed in recombinant microorganisms, and thus can be used in various assays, including enzyme immunoassay.

Abstract: The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.