Abstract: A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

Abstract: A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

Abstract: A micro-needle device is applicable for a target tissue with a first area having a first parameter and a second area having a second parameter. The micro-needle device includes a substrate and a micro-needle array connected to the substrate. The micro-needle array includes a first micro-needle corresponding to the first area and a second micro-needle corresponding to the second area. One of the surfaces of the substrate having the micro-needle array corresponds to the surface curvature of the target tissue. The first micro-needle has a first configuration corresponding to the first parameter, and the second micro-needle has a second configuration corresponding to the second parameter and different from the first configuration. The configuration includes at least one selected from a group consisting of length, distribution density, diameter, and medicine-carrying amount of micro-needle. A method for manufacturing a micro-needle device and a method for manufacturing a micro-needle mold are also provided.

Abstract: A method for producing image of biological sample, including steps: inputting a grayscale reflection image or a grayscale interference image of a biological sample into a first memory block; inputting a grayscale fluorescent image of the biological sample into a second memory block; using an information processing apparatus to convert the grayscale reflection image or the grayscale interference image into an RGB reflection image or an RGB interference image via first color transform operation and using the information processing apparatus to convert the grayscale fluorescent image into an RGB fluorescent image via second color transform operation; using the information processing apparatus to perform an image fusion operation and an intensity inversion operation on the RGB reflection image and the RGB fluorescent image or on the RGB interference image and the RGB fluorescent image to generate a pseudo H&E image; and outputting the pseudo H&E image to a display unit.