Abstract: A method for producing liposomes entrapping Cyclosporine A (CsA). The method comprising preparing a suspension of CsA-free liposomes with a pH level of 7, preparing a mixture with a pH level of 7 by adding the suspension of CsA-free liposomes in the sucrose phosphate buffer to an ethanolic solution of CsA, and forming the liposomes entrapping CsA by incubating the mixture at a temperature level between 42° C. and 48° C. for a time duration between 55 and 65 minutes.