Abstract: The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

Abstract: Geologic modeling methods and systems may use design-space to design-space mapping to facilitate simulation grid generation for multiple interpretations of a subsurface region. As one example, one or more embodiments of a geologic modeling method may comprise: obtaining first and second geologic models having different structural interpretations of a subsurface region; mapping each of the geologic models to associated design space models representing an unfaulted subsurface region; determining a design-to-design space mapping from the first design space model to the second design space model; using said mapping to copy parameter values from the first design space model to the second of the design space model; gridding each of the design space models to obtain design space meshes; partitioning cells in the first and second design space meshes along faults; reverse mapping the partitioned design space meshes to the physical space to obtain first and second physical space simulation meshes.

Abstract: An array substrate and a display panel. The array substrate includes a thin film transistor array layer including a driving transistor, a switching transistor, and a capacitor. The driving transistor includes a first active layer, a first gate insulating layer, a first gate, and an insulating dielectric layer sequentially stacked. The switching transistor includes a second active layer, a second gate insulating layer, and a second gate sequentially stacked. The insulating dielectric layer and the second gate insulating layer are located at a same layer. A thickness of the first gate insulating layer is greater than a thickness of the second gate insulating layer. The capacitor includes a first electrode plate and a second electrode plate. The first electrode plate and the first gate are disposed on same layer, and the second electrode plate and the second gate are disposed on same layer.

Abstract: A modified nucleoside or nucleotide, the 3?-OH of the modified nucleoside or nucleotide being reversibly blocked; meanwhile, the present invention also relates to a kit comprising the nucleoside or nucleotide, and a sequencing method based on the nucleoside or nucleotide.

Abstract: The presently claimed invention provides an electrochemical analytical apparatus for electrochemical bath analysis. The apparatus comprise a static electrode and a rotatable unit. As steady liquid flow can be generated on the electrolytic surface of the static electrode by the rotatable unit through rotation, the static disk electrode does not involve any movement during the bath analysis such that the design of the electrical contact in the electrode can be substantially simplified.

Abstract: A method for filling a through hole (TH) located on a substrate is provided. The TH is a continuous channel having an upper rim, a lower rim and an interior surface. In one embodiment, the method comprises steps (a)-(d). In the step (a), a conductive material (CM) is deposited over the substrate to thereby deposit a layer of the CM around the rims and on the interior surface. In the step (b), the deposited CM is etched. In particular, the etching step selectively removes more CM deposited at the rims relative to CM deposited at a mid-section of the interior surface of the channel. In the step (c), the steps (a) and (b) are optionally repeated until the channel is sealed at the mid-section by a bridge formed of CM. In the step (d), the CM is further deposited over the substrate to thereby completely fill the TH.

Abstract: The presently claimed invention provides an electrochemical analytical apparatus for electrochemical bath analysis. The apparatus comprise a static electrode and a rotatable unit. As steady liquid flow can be generated on the electrolytic surface of the static electrode by the rotatable unit through rotation, the static disk electrode does not involve any movement during the bath analysis such that the design of the electrical contact in the electrode can be substantially simplified.