Abstract: The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

Abstract: The present invention relates to fields of clinical application of nerve defect repair and the medical three-dimensional (3D) printing technology, and provides an integrated visualization method for three-dimensional (3D) reconstruction of internal structure of human peripheral nerves. The method comprises the following steps: obtaining human peripheral nerves, preparing nerve specimens ex vivo by staining with an iodine preparation in combination with a freeze-drying method; scanning the pretreated peripheral nerves using Micro CT to acquire lossless two-dimensional images, and performing binarization processing to the two-dimensional images, then conducting image segmentation based on textural features to acquire images of nerve fascicles; finally, reconstructing the segmented images into a visualization model by using a supercomputer.

Abstract: The present invention relates to fields of clinical application of nerve defect repair and the medical three-dimensional (3D) printing technology, and provides an integrated visualization method for three-dimensional (3D) reconstruction of internal structure of human peripheral nerves. The method comprises the following steps: obtaining human peripheral nerves, preparing nerve specimens ex vivo by staining with an iodine preparation in combination with a freeze-drying method; scanning the pretreated peripheral nerves using Micro CT to acquire lossless two-dimensional images, and performing binarization processing to the two-dimensional images, then conducting image segmentation based on textural features to acquire images of nerve fascicles; finally, reconstructing the segmented images into a visualization model by using a supercomputer.

Abstract: An organic electroluminescent device comprises a lower electrode provided on the surface of a color filter, wherein the lower electrode is directly provided on the surface of an overcoat layer. An isolation layer is further provided between the lower electrode and the overcoat layer, such that the connection between the lower electrode and the color filter can be proceeded beneficially. Besides, the situation of barrier layer broken will be not happened. Thus, the structure completeness of the organic electroluminescent device can be held, and the production yield of that can be improved.

Abstract: The present invention provides an electric shielding and anti-glaring multi-layer coating for cathode ray tube, and the method for making the same. A plurality of sputtering layers are formed on the outer surface of the glass panel of the CRT. An anti-glaring layer is formed on the outer surface of the sputtering layers by spraying. The plurality of sputtering layers contains conductive material such as ITO and having a protective layer to protect the conductive layer from acid corrosion. The multi-layer coating also has anti-reflection property in wide spectral range, and also has good hardness and resistance to fingerprint.