Abstract: This invention relates to recombinant nucleic constructs comprising a sequence-specific DNA binding protein, DNA-dependent DNA polymerase and a DNA encoded repair template, optionally a DNA endonuclease or wherein the sequence-specific DNA binding protein comprises DNA endonuclease activity, and methods of use thereof for modifying nucleic acids in cells and organisms.

Abstract: Described herein are recruitment methods and compounds, compositions, and systems for recruitment. Such methods and compounds, compositions, and systems include the use of fusion proteins of Agrobacterium effector proteins, recruiting motifs, and/or nucleic acid binding polypeptides. Also described herein are methods and compositions for template editing of genomic DNA using Agrobacterium-derived T-DNA molecules and/or proteins.

Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: Described herein are recruitment methods and compounds, compositions, and systems for recruitment. Also described herein are methods and compositions for template editing of genomic DNA using Agrobacterium-derived T-DNA molecules and/or proteins.

Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: Described herein are color-based and/or visual methods for identifying the presence of a transgene (e.g., the presence of a transgene in a cell, seed, plant part, and/or plant) along with composition, systems, and constructs relating to the same.

Abstract: This invention relates to recombinant nucleic constructs comprising CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: This invention relates to recombinant nucleic constructs comprising a sequence-specific DNA binding protein, DNA-dependent DNA polymerase and a DNA encoded repair template, optionally a DNA endonuclease or wherein the sequence-specific DNA binding protein comprises DNA endonuclease activity, and methods of use thereof for modifying nucleic acids in cells and organisms

Abstract: Described herein are recruitment methods and compounds, compositions, and systems for recruitment. Also described herein are methods and compositions for template editing of genomic DNA using Agrobacterium-derived T-DNA molecules and/or proteins.

Abstract: This invention relates to recombinant nucleic constructs comprising a DNA binding domain, an endonuclease and a reverse transcriptase and methods of use thereof for modifying nucleic acids in plants.

Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.

Abstract: Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe clonal diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe clonal diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the male diploid genome in the zygote and normal development of the endosperm.

Abstract: Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the female diploid genome in the zygote and normal development of the endosperm.

Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include promoter sequences with direct expression in an egg cell or embryonic cell-preferred manner. Such compositions find use in, for example, a method for expressing a heterologous nucleotide sequence in a plant; detection of specific cell types in the ovule and targeted ablation of specific cell types.