Abstract: The invention described holds dialysis bag and other similar articles containing delicate membrane securely in place so that biological solution can be added or collected, safely. The Dialysis Bag Holder contains bottom, vertical and top plates, cradle tubes and a funnel holding mechanism. The bottom plate holds vertical plate and cradle tubes. The vertical plate, besides providing additional support to cradle tubes, holds the top plate firmly in place. The top plate located over the tip of cradle tubes, seats funnel for pouring large volume of sample. Cradle tubes, attached permanently at an angle to the top of bottom plate, hold dialysis bags. The angle of attachment makes cradle tubes have an ascending slope. The concave holding surface of cradle tube complements with the cylindrical body of the bag or similar articles. The entire unit provides stability to the dialysis bag before, during and after loading biological sample.