Abstract: A method of measuring optical properties of a specimen includes generating illumination light at a plurality of illumination wavelengths and, for each of the plurality of illumination wavelengths, directing the illumination light to impinge on the specimen, collecting sample light passing through the specimen, and detecting the collected sample light using a polarization state analyzer to form a set of polarization channels. The method also includes receiving a calibration tensor, converting the set of polarization channels for each of the illumination wavelengths into Stokes parameter maps using the calibration tensor, denoising the Stokes parameter maps, and deconvolving the Stokes parameter maps to provide density, anisotropy, and orientation measurements of the specimen. The method can multiplex intrinsic density, anisotropy, and orientation measurements of the specimen and density, anisotropy, and orientation measurements of labeled fluorescent molecules.

Abstract: A method of measuring optical properties of a specimen includes generating illumination light at a plurality of illumination wavelengths and, for each of the plurality of illumination wavelengths, directing the illumination light to impinge on the specimen, collecting sample light passing through the specimen, and detecting the collected sample light using a polarization state analyzer to form a set of polarization channels. The method also includes receiving a calibration tensor, converting the set of polarization channels for each of the illumination wavelengths into Stokes parameter maps using the calibration tensor, denoising the Stokes parameter maps, and deconvolving the Stokes parameter maps to provide density, anisotropy, and orientation measurements of the specimen. The method can multiplex intrinsic density, anisotropy, and orientation measurements of the specimen and density, anisotropy, and orientation measurements of labeled fluorescent molecules.

Abstract: A method of measuring optical properties of a specimen, for example, a uniaxial specimen, includes generating a plurality of illumination patterns incident on the specimen and, for each of the plurality of illumination patterns, collecting sample light passing through the specimen and detecting the collected sample light using a polarization state analyzer to form a set of polarization channels. The method also includes receiving a calibration tensor, converting the set of polarization channels for each of the illumination patterns into Stokes parameter maps using the calibration tensor, and deconvolving the Stokes parameter maps to provide volumetric measurement of permittivity tensor of the specimen, specifically, absorption, optical path length, optical anisotropy, and 3D orientation of the specimen.