Abstract: Methods of promoting healing of a wound in a dermal location of a subject are provided. Aspects of the methods may include administering an effective amount of a YAP inhibitor composition to the wound to modulate mechanical activation of Engrailed-1 lineage-negative fibroblasts (ENFs) in the wound to promote ENF-mediated healing of the wound. Also provided are methods of preventing scarring during healing of a wound in a subject and methods of promoting hair growth on a subject. Aspects of the methods may include forming a wound in a dermal location of a subject and administering an effective amount of a YAP inhibitor composition to the wound to modulate mechanical activation of Engrailed-1 lineage-negative fibroblasts (ENFs) in the wound to promote ENF-mediated healing of the wound. Also provided are kits including an amount of a YAP inhibitor composition and a tissue disrupting device.

Abstract: A system and method for analyzing a plurality of tissue image samples at different stages of image processing in order to establish the level of fibrosis within a patient from which the tissue image sample was retrieved. Various embodiments incorporate machine learning techniques which enable an automated progressive approach to effectively and efficiently diagnosing fibrotic conditions based on tissue image samples.