Patents by Inventor Shana O. Kelley
Shana O. Kelley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160077046Abstract: Contemplated methods and devices comprise performing electrochemical sample analysis in a multiplexed electrochemical detector having reduced electrical cross-talk. The electrochemical detector includes electrodes that share a common lead from a plurality of leads. The sample, which may be a liquid sample, is introduced into one or more sample wells and a signal is applied to at least one of the electrodes. A response signal is measured while simultaneously applying a substantially fixed potential to each of a remainder of the plurality of leads.Type: ApplicationFiled: November 30, 2015Publication date: March 17, 2016Inventors: Shana O. Kelley, Edward Hartley Sargent, Brian Lam
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Publication number: 20150377831Abstract: Digital microfluidic devices, and methods for the control and fabrication thereof, and provided in which an integrated nanostructured electrodeposited electrode is provided such that the digital microfluidic array can be actuated to contact a droplet with the nanostructured electrodeposited electrode. In some embodiments, digital microfluidic devices are provided having an integrated electrochemical sensor, where the working electrode is provided in the form of a nanostructured electrodeposited electrode. Various methods of fabricating such integrated device are described, including methods that employ a lift-off process that exposes an underlying base electrode for the electrodeposition of a nanostructured electrodeposited electrode, while providing a hydrophobic surface for droplet transport.Type: ApplicationFiled: June 26, 2015Publication date: December 31, 2015Inventors: Aaron R. Wheeler, Shana O. Kelley, Darius G. Rackus, Michael D.M. Dryden, Brian Lam
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Patent number: 9217179Abstract: Contemplated methods and devices comprise performing electrochemical sample analysis in a multiplexed electrochemical detector having reduced electrical cross-talk. The electrochemical detector includes electrodes that share a common lead from a plurality of leads. The sample, which may be a liquid sample, is introduced into one or more sample wells and a signal is applied to at least one of the electrodes. A response signal is measured while simultaneously applying a substantially fixed potential to each of a remainder of the plurality of leads.Type: GrantFiled: March 15, 2013Date of Patent: December 22, 2015Assignee: The Governing Council of the University of TorontoInventors: Shana O. Kelley, Edward H. Sargent, Brian Lam
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Publication number: 20140342359Abstract: Contemplated methods and devices comprise use of a charged probe and a neutralizer in the electrochemical detection of a wide range of analytes, including nucleic acids, proteins, and small molecules. In certain embodiments the neutralizer forms a complex with the probe that has a reduced charge magnitude compared to the probe itself, and is displaced from the probe when the complex is exposed to the analyte.Type: ApplicationFiled: November 21, 2012Publication date: November 20, 2014Inventors: Shana O. Kelley, Alexandre Zaragoza, Edward Hartley Sargent, Jagotamoy Das, Kristin Cederquist
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Publication number: 20140170646Abstract: Contemplated methods and devices are drawn to preparation and analysis of analytes from biological samples. In a preferred embodiment the analytes are nucleic acids that are both released from biological compartment present in the sample and fragmented through the use of a voltage potential applied to a pair of electrodes. The nucleic acids thus prepared are subsequently characterized.Type: ApplicationFiled: March 12, 2012Publication date: June 19, 2014Applicant: Xagenic, Inc.Inventors: Shana O. Kelley, Susan Bortolin, Reginald James McKenzie Orton, Stefan Christopher Wiechula
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Publication number: 20140087375Abstract: Disclosed are biomolecule based bioprobes that exhibit improved water solubility and monolayer-forming properties with substantially little or no aggregation that can appreciably interfere with binding of the bioprobes to a target nucleotide. The bioprobes may be used in conjunction with a suitable reporter system to detect very small quantities of biological markers. The bio-probes comprise a nucleobase sequence capable of hybridizing to a target nucleotide; and at least one charged functional group attached to said nucleobase sequence. Also disclosed are biosensors, and sensing devices that comprise the bio-probe. Further disclosed are suitable electrochemical reporter systems for use with bioprobes. Methods of use of these devices and probes, including for the detection of target biomarkers, including biomarkers for cancer cells or pathogens, are also included.Type: ApplicationFiled: February 6, 2012Publication date: March 27, 2014Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTOInventors: Shana O. Kelley, Zhichao Fang, Elizaveta Vasilyeva
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Publication number: 20140072962Abstract: Contemplated methods and devices comprise detecting the presence of a pathogen in a biological host. In certain implementations, a sample is provided from a biological host. A biosensor is provided, the biosensor having a first probe configured to detect a control marker in the sample, the control marker being an endogenous element of the biological host. The biosensor has a second probe configured to detect the presence of a target marker in the sample, the target marker being from a pathogen in the biological host. The sample is applied to the biosensor, and the presence or absence of the control marker in the sample is identified using the first probe. The presence or absence of the target marker in the sample is identified using the second probe.Type: ApplicationFiled: August 9, 2013Publication date: March 13, 2014Applicant: Xagenic Inc.Inventors: Shana O. Kelley, Graham Jack
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Publication number: 20140005068Abstract: Described herein are systems and methods for detecting a target analyte in a sample with electrodes, comprising a linker and an antibody attached to the linker, and measuring an electrocatalytic signal changes generated by binding of an analyte in the sample to the antibody. Also disclosed herein are kits for electrochemical detection of protein analytes.Type: ApplicationFiled: January 11, 2012Publication date: January 2, 2014Applicant: The Governing Council of the University of TorontoInventors: Jagotamoy Das, Shana O. Kelley
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Publication number: 20130316340Abstract: Contemplated methods and devices comprise performing electrochemical sample analysis in a multiplexed electrochemical detector having reduced electrical cross-talk. The electrochemical detector includes electrodes that share a common lead from a plurality of leads. The sample, which may be a liquid sample, is introduced into one or more sample wells and a signal is applied to at least one of the electrodes. A response signal is measured while simultaneously applying a substantially fixed potential to each of a remainder of the plurality of leads.Type: ApplicationFiled: March 15, 2013Publication date: November 28, 2013Inventors: Shana O. Kelley, Edward H. Sargent, Brian Lam
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Publication number: 20090270266Abstract: The present invention provides a method of detecting an analyte in a sample with probe-modified electrodes and measuring an electrocatalytic signal generated by a binding of an analyte in the sample to a probe.Type: ApplicationFiled: April 12, 2006Publication date: October 29, 2009Inventors: Shana O. Kelley, Marcel Roberts
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Patent number: 7361470Abstract: The detection of specific DNA sequences using electrochemical readout would permit the rapid and inexpensive detection and identification of bacterial pathogens and the analysis of human genes. A new assay developed for this purpose is described that harnesses an electrocatalytic process to monitor DNA hybridization.Type: GrantFiled: November 11, 2005Date of Patent: April 22, 2008Assignee: Trustees of Boston CollegeInventors: Shana O. Kelley, Melissa Lapierre-Devlin, Meaghan O'Keefe
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Patent number: 7202037Abstract: Compositions and methods for electrochemical detection and localization of genetic point mutations, common DNA lesions and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential.Type: GrantFiled: August 14, 2003Date of Patent: April 10, 2007Assignee: California Institute of TechnologyInventors: Jacqueline K. Barton, Elizabeth M. Boon, Shana O. Kelley, Michael G. Hill
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Patent number: 6958216Abstract: A class of biological sensing devices that include a substrate comprising an array of carbon nanotubes (CNTs) to which are chemically attached biological molecules is disclosed. The attached biological molecules are capable of electrical conductivity that is responsive to chemical changes occurring as a result of their interaction with target species. A means for means for using DNA as a material of potential in molecular electronic sensor devices, being primarily based on molecular electron-transfer reaction processes between DNA-binding donors and acceptors is also disclosed, including composition, method of manufacture and their use are described.Type: GrantFiled: January 9, 2002Date of Patent: October 25, 2005Assignee: The Trustees of Boston CollegeInventors: Shana O. Kelley, John Fourkas, Michael Naughton, Zhifeng Ren
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Publication number: 20040063126Abstract: Compositions and methods for electrochemical detection and localization of genetic point mutations, common DNA lesions and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential.Type: ApplicationFiled: August 14, 2003Publication date: April 1, 2004Inventors: Jacqueline K. Barton, Elizabeth M. Boon, Shana O. Kelley, Michael G. Hill
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Patent number: 6649350Abstract: Compositions and methods for electrochemical detection and localization of genetic point mutations, common DNA lesions and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential.Type: GrantFiled: September 13, 2001Date of Patent: November 18, 2003Assignee: California Institute of TechnologyInventors: Jacqueline K. Barton, Elizabeth M. Boon, Shana O. Kelley, Michael G. Hill
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Publication number: 20020172963Abstract: A class of biological sensing devices that include a substrate comprising an array of carbon nanotubes (CNTs) to which are chemically attached biological molecules is disclosed. The attached biological molecules are capable of electrical conductivity that is responsive to chemical changes occurring as a result of their interaction with target species. A means for means for using DNA as a material of potential in molecular electronic sensor devices, being primarily based on molecular electron-transfer reaction processes between DNA-binding donors and acceptors is also disclosed, including composition, method of manufacture and their use are described.Type: ApplicationFiled: January 9, 2002Publication date: November 21, 2002Inventors: Shana O. Kelley, John Fourkas, Michael Naughton, Zhifeng Ren
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Publication number: 20020146716Abstract: Compositions and methods for electrochemical detection and localization of genetic point mutations, common DNA lesions and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential.Type: ApplicationFiled: September 13, 2001Publication date: October 10, 2002Inventors: Jacqueline K. Barton, Elizabeth M. Boon, Shana O. Kelley, Michael G. Hill
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Patent number: 6461820Abstract: Compositions and methods for electrochemical detection and localization of genetic point mutations and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential.Type: GrantFiled: December 29, 2000Date of Patent: October 8, 2002Assignee: California Institute of TechnologyInventors: Jacqueline K. Barton, Michael G. Hill, Shana O. Kelley
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Publication number: 20020055103Abstract: Compositions and methods for electrochemical detection and localization of genetic point mutations and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential.Type: ApplicationFiled: December 29, 2000Publication date: May 9, 2002Inventors: Jacqueline K. Barton, Michael G. Hill, Shana O. Kelley
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Patent number: 6221586Abstract: Compositions and methods for electrochemical detection and localization of genetic point mutations and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential.Type: GrantFiled: April 8, 1998Date of Patent: April 24, 2001Assignee: California Institute of TechnologyInventors: Jacqueline K. Barton, Michael G. Hill, Shana O. Kelley