Patents by Inventor Shanavaz Nasarabadi

Shanavaz Nasarabadi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7972818
    Abstract: Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5? end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM™ on the 3? end of the reporting DNA sequence and a quencher dye, e.g., TAMRA™, on the 5? end.
    Type: Grant
    Filed: June 16, 2006
    Date of Patent: July 5, 2011
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: Shanavaz Nasarabadi, Richard G. Langlois, Kodumudi S. Venkateswaran
  • Patent number: 7867713
    Abstract: A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.
    Type: Grant
    Filed: April 21, 2008
    Date of Patent: January 11, 2011
    Assignee: Lawrence Livermore National Security, LLC
    Inventor: Shanavaz Nasarabadi
  • Publication number: 20090263794
    Abstract: A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.
    Type: Application
    Filed: April 21, 2008
    Publication date: October 22, 2009
    Inventor: Shanavaz Nasarabadi
  • Publication number: 20070117110
    Abstract: Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5? end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3? end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5? end.
    Type: Application
    Filed: June 16, 2006
    Publication date: May 24, 2007
    Inventors: Shanavaz Nasarabadi, Richard Langlois, Kodumudi Venkateswaran
  • Publication number: 20070107539
    Abstract: A system for sampling air and collecting particles entrained in the air that potentially include bioagents. The system comprises providing a receiving surface, directing a liquid to the receiving surface and producing a liquid surface. Collecting samples of the air and directing the samples of air so that the samples of air with particles entrained in the air impact the liquid surface. The particles potentially including bioagents become captured in the liquid. The air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid but cause minor turbulence. The liquid surface has a surface tension and the collector samples the air and directs the air to the liquid surface so that the air with particles entrained in the air impacts the liquid surface with sufficient velocity to entrain the particles into the liquid, but cause minor turbulence on the surface resulting in insignificant evaporation of the liquid.
    Type: Application
    Filed: August 1, 2006
    Publication date: May 17, 2007
    Inventors: Perry Bell, Allen Christian, Christopher Bailey, Ladona Willis, Donald Masquelier, Shanavaz Nasarabadi
  • Publication number: 20070107537
    Abstract: A system for sampling air and collecting particles entrained in the air comprising a receiving surface, a liquid input that directs liquid to the receiving surface and produces a liquid surface, an air input that directs the air so that the air with particles entrained in the air impact the liquid surface, and an electrostatic contact connected to the liquid that imparts an electric charge to the liquid. The particles potentially including bioagents become captured in the liquid by the air with particles entrained in the air impacting the liquid surface. Collection efficiency is improved by the electrostatic contact electrically charging the liquid. The effects of impaction and adhesion due to electrically charging the liquid allows a unique combination in a particle capture medium that has a low fluid consumption rate while maintaining high efficiency.
    Type: Application
    Filed: August 1, 2006
    Publication date: May 17, 2007
    Inventors: Perry Bell, Allen Christian, Christopher Bailey, Ladona Willis, Donald Masquelier, Shanavaz Nasarabadi
  • Publication number: 20070107538
    Abstract: A system for sampling air and collecting particles potentially including bioagents entrained in the air for detection. The system comprises collecting a sample of the air with the particles entrained in the air, directing the sample to a receiving surface, directing a liquid to the receiving surface thereby producing a liquid surface, wherein the particles potentially including bioagents become captured in the liquid, and heating the liquid wherein the particles potentially including bioagents become heated to lysis the bioagents.
    Type: Application
    Filed: August 1, 2006
    Publication date: May 17, 2007
    Inventors: Perry Bell, Allen Christian, Christopher Bailey, Ladona Willis, Donald Masquelier, Shanavaz Nasarabadi
  • Patent number: 7083951
    Abstract: Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5? end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3? end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5? end.
    Type: Grant
    Filed: February 14, 2002
    Date of Patent: August 1, 2006
    Assignee: The Regents of the University of California
    Inventors: Shanavaz Nasarabadi, Richard G. Langlois, Kodumudi S. Venkateswaran
  • Publication number: 20060057599
    Abstract: An autonomous monitoring apparatus for monitoring air, water, soil, or other substance for bioagents. A collector gathers a quantity of the air, water, soil, or other substance being monitored. A sample preparation system prepares a sample of the selected potential bioagent particles. The sample is analyzed by a system for detecting said bioagents.
    Type: Application
    Filed: November 29, 2004
    Publication date: March 16, 2006
    Inventors: John Dzenitis, Steve Brown, Billy Colston, Dora Gutierrez, Bruce Henderer, Benjamin Hindson, Ramakrishna Madabhushi, Anthony Makarewicz, Don Masquelier, Mary McBride, Thomas Metz, Shanavaz Nasarabadi, Ujwal Setlur, Sally Smith, Kodumudi Venkateswaran
  • Publication number: 20050239192
    Abstract: A nucleic acid assay system for analyzing a sample using a reagent. A sample and reagent delivery unit is operatively connected to a thermal cycler for delivering the sample and the reagent to the thermal cycler. A hybridization chamber is operatively connected to the thermal cycler. A flow cytometer is operatively connected to the hybridization chamber.
    Type: Application
    Filed: June 21, 2005
    Publication date: October 27, 2005
    Inventors: Shanavaz Nasarabadi, Richard Langlois, Billy Colston, Evan Skowronski, Fred Milanovich
  • Publication number: 20020155482
    Abstract: Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5′ end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3′ end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5′ end.
    Type: Application
    Filed: February 14, 2002
    Publication date: October 24, 2002
    Inventors: Shanavaz Nasarabadi, Richard G. Langlois, Kodumudi S. Venkateswaran