Abstract: An isolated or a purified nucleic acid sequence for enhancing expression levels of a protein of interest, in 5? to 3? direction, comprises: a cytomegalovirus (CMV) promoter; an eEF-1 intron repeat (eEF-1 IR); and a regular sequence, which comprises: at least one tag element, a fixable linker sequence, wherein the fixable sequence is TEV sequence; and a multiple cloning site (MCS). By means of the array of the specific promoter and eEF-1 IR, the expression and purity of the recombinant protein could be enhanced; wherein eEF-1 IR can reduce the length of vector and assist RNA polymerase II transcription. Besides, TEV sequence of the fixable linker sequence of the regular sequence can remove a tag on recombinant protein; a specific target can be inserted into the multiple cloning site.

Abstract: An isolated or a purified nucleic acid sequence for enhancing expression levels of a protein of interest, in 5? to 3? direction, comprises: a cytomegalovirus (CMV) promoter; an eEF-1 intron repeat (eEF-1 IR); and a regular sequence, which comprises: at least one tag element, a fixable linker sequence, wherein the fixable sequence is TEV sequence; and a multiple cloning site (MCS). By means of the array of the specific promoter and eEF-1 IR, the expression and purity of the recombinant protein could be enhanced; wherein eEF-1 IR can reduce the length of vector and assist RNA polymerase II transcription. Besides, TEV sequence of the fixable linker sequence of the regular sequence can remove a tag on recombinant protein; a specific target can be inserted into the multiple cloning site.

Abstract: A method of processing application security for uses in a platform-as-a-service layer (PAAS layer) includes steps as follows. First, an application program is scanned to find out a vulnerable code segment. Then, when the vulnerable code segment isn't fixed through a security process, a secure code segment is weaved into this unfixed vulnerable code segment, so as to ensure the security of the application program. Moreover, a system of processing application security is also disclosed in specification.

Abstract: A cloud-based test system is disclosed. The cloud-based test system utilizes several cloud systems for testing. Each cloud system includes several cloud servers for providing a cloud resource to simulate several virtual test machines. The cloud-based test system includes several slave servers and a main server. Each slave server corresponds to one of the cloud systems for controlling the corresponding virtual test machines. The main server receives a test instruction, which is utilized to execute a target test item for a target electrical device, from a client, and generates a test environment condition corresponding to the test instruction. The main server determines the virtual test machines for executing the target test item and the at least one server to control the virtual test machines. The main server transmits the test instruction and the corresponding test environment condition to the server slave servers for testing.

Abstract: A cloud-based test system is disclosed. The cloud-based test system utilizes several cloud systems for testing. Each cloud system includes several cloud servers for providing a cloud resource to simulate several virtual test machines. The cloud-based test system includes several slave servers and a main server. Each slave server corresponds to one of the cloud systems for controlling the corresponding virtual test machines. The main server receives a test instruction, which is utilized to execute a target test item for a target electrical device, from a client, and generates a test environment condition corresponding to the test instruction. The main server determines the virtual test machines for executing the target test item and the at least one server to control the virtual test machines. The main server transmits the test instruction and the corresponding test environment condition to the server slave servers for testing.

Abstract: A method for assessing a risk of suffering from a gout of a subject is provided. The method includes steps of obtaining a nucleotide sample from the subject; determining a genetic polymorphism of one of a Urate transporter 1 (URAT1) gene and an alpha-kinase 1 (ALPK1) gene in the nucleotide sample, wherein the genetic polymorphism is associated with an occurrence of the gout; and comparing the genetic polymorphism with a predetermined genetic polymorphism so as to assess the risk of suffering from the gout of the subject.