Abstract: Molecular markers of response to various chemotherapies were measured in tumor samples of 5 sarcoma subtypes to identify effective treatment regimens based on expression of chemotherapy biomarkers. The molecular profiles for rhabdomyosarcoma support the use of standard-of-care treatment, while other profiles showed that alternative agents differing from standard-of-care will be effective, including gemcitabine (osteosarcoma), taxane (leiomyosarcoma, Ewing's sarcoma), and platinum (leiomyosarcoma, liposarcoma, and osteosarcoma).