Abstract: An interference ribonucleic acid containing specific sequence for inhibiting MMP1, which is composed of 19 to 25 nucleotides, wherein the interference ribonucleic acid has a sequence selected from the group consisted of the sequences as set forth in, or essentially identical to, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10. By means of the interference ribonucleic acid and MMP1 with high specificity binding, gene and protein expression of endogenous MMP1 of cancer cell can be inhibited, and then the interference ribonucleic acid can be used for pharmaceutical composition to prevent or treat cancer metastasis or to improve wrinkle resistance.

Abstract: The present invention provides a method of producing chicken essence powder, comprising hydrolyzing the chicken raw material by using the composite protease combining alkaline protease from Bacillus licheniformis with neutral protease from Bacillus subtilis, and spray drying a chicken essence concentration to obtain the chicken essence powder. In addition, the present invention also provides a chicken essence powder produced by the aforementioned method. The chicken essence powder has a particle size ranging from 50 ?m to 100 ?m, a molecular weight ranging from 200 Da to 2500 Da, and an amount of cholesterol less than 3 milligrams per 100 grams of chicken essence powder. In addition, the present invention also provides a method for scavenging ?,?-diphenyl-?-picrylhydrazyl (DPPH) radical by administering the chicken essence powder to a host in need thereof.

Abstract: An E. coli expression system for producing mature human tyrosinase is provided and includes an E. coli host, which has a trait for expressing endogenous methionyl aminopeptidase in cytoplasm, and an expression vector transformed into the E. coli host. The expression vector has a replication origin sequence of an E. coli, and the expression vector includes an inducible promoter and a DNA fragment of human tyrosinase having a sequence referenced as SEQ ID NO:3, wherein a 5? end of the DNA fragment is constructed at a restriction enzyme NdeI recognition site located at the downstream of the inducible promoter.

Abstract: The present invention provides a mineral-peptide chelate comprising a peptide consisting of 2˜18 amino acids and a mineral chelated to the peptide, wherein the peptide can be a hydrolysate obtained by hydrolyzing soybean or other protein materials with proteases, or a product obtained by hydrolyzing soybean or other protein material with proteases and fermentation. The mineral-peptide chelate of the present invention may further comprise a carrier which covers the peptide and the mineral which is chelated to the peptide.

Abstract: The present invention relates to a purified sulfite reductase which has the following characteristics: a. It functions to catalyze the reduction of sulfites to sulfides or to recover the sulfhydryl groups from disulfide groups, b. In the aforesaid catalysis of the reduction, reduced nicotinamide dinucleotide phosphate (NADPH), methyl viologen (MVH) or other donors acts as an electron donor, c. Its molecular weight is from 100,000 to 400,000, d. The optimal temperature for its activity is from 20° C. to 30° C., and e. The optimal pH for its activity is from 6.5 to 8.0. The present invention also relates to a process for producing the purified sulfite reductase, and a method for recovering the proteins of denatured fish by using said sulfite reductase in solution or powder form.

Abstract: The present invention relates to a N-glycosylation-modified recombinant chicken cystatin, characterized in that Asn106-Ile108 in its amino acid sequence is modified to Asn106-Thr108. The present invention also relates to a method for producing said N-glycosylation-modified recombinant chicken cystatin, wherein a site-directed mutated cDNA encoding chicken cystatin is cloned in an expression vector, with which a yeast strain is in turn transformed, and the yeast transformant is then cultured for producing a recombinant chicken cystatin wherein the Asn residue in the Asn106-Thr108 of its amino acid sequence that is produced by said site-directed mutagenesis can be modified by N-glycosylation. The N-glycosylation-modified recombinant chicken cystatin disclosed in the present invention has improved stability in a freezing-thawing process and in a heating process.

Abstract: The present invention relates to a N-glycosylation-modified recombinant chicken cystatin, characterized in that Asn106-Ile108 in its amino acid sequence is modified to Asn106-Thr108. The present invention also relates to a method for producing said N-glycosylation-modified recombinant chicken cystatin, wherein a site-directed mutated cDNA encoding chicken cystatin is cloned in an expression vector, with which a yeast strain is in turn transformed, and the yeast transformant is then cultured for producing a recombinant chicken cystatin wherein the Asn residue in the Asn106-Thr108 of its amino acid sequence that is produced by said site-directed mutagenesis can be modified by N-glycosylation. The N-glycosylation-modified recombinant chicken cystatin disclosed in the present invention has improved stability in a freezing-thawing process and in a heating process.

Abstract: The present invention relates to novel strains Pediococcus pentosaceus YJL with the accession numbers FERM-BP 8450 (BCRC 910210) and Pediococcus pentosaceus YJS with the accession numbers FERM-BP 8449 (BCRC 910211). The present invention also provides a method for processing fish foodstuffs comprising using lactic acid bacteria and the fish foodstuffs obtained thereby. The present invention also provides a method for processing legume foodstuffs comprising using lactic acid bacteria and the legume foodstuffs obtained thereby. The present invention further provides bacteriocins produced from the strains. The present methods are capable of producing processed products with reduced growth of unwanted microorganisms, improved flavor and enhanced economic value. The pentocins are effective on the inhibition of unwanted microorganisms thereby insuring the foodstuff a good quality during storage.