Abstract: A prosthetic heart valve provided herein can include a cultured cell tissue leaflet. In some cases, a prosthetic heart valve can include a plurality of leaflets secured together and retained within the expandable tubular member. The cultured cell tissue can be obtained by culturing fibroblast cells, smooth muscle cells, or a combination thereof to form a sheet of cultured cells and chemically cross-linking the fibroblast cells while under tension. In some cases, the cultured cell tissue can be radially tensioned. In some cases, the cultured cell tissue can be bi-axially tensioned.

Abstract: A cell filtration assembly adapted to capture cells from a biological sample during centrifugation includes a centrifugation tube, a cell collection device adapted to be secured within a tapered end of the centrifugation tube and a funnel structure adapted to direct fluid into the cell collection device. The cell collection device includes a rectilinear structure that is adapted to permit fluid to flow through the rectilinear structure and a cell capture surface that is secured relative to the rectilinear structure. The cell collection device may be sectioned in either a horizontal or vertical orientation.

Abstract: A tissue filtration device may include an elongate hollow needle assembly having open distal and proximal ends. In some cases, the tissue filtration device may include a collection device containing a filter material and coupled to the elongate hollow needle assembly and in fluid communication with the elongate hollow needle. Also included may be a suction assembly coupled to the collection device and configured to draw fluid from the elongate hollow needle assembly into the collection device.

Abstract: A cell filtration assembly adapted to capture cells from a biological sample during centrifugation includes a filter support member including a sample well and a filter membrane that spans the sample well. In some cases, the filter support member and/or the filter membrane may be adapted to be sectioned. In some cases, a protective element may be disposed over at least a portion of the filter support member in order to protect the filter support member from tissue processing reagents during processing of the cells captured from the biological sample.

Abstract: Filtration devices and methods for collecting, filtering, and/or processing biological samples are disclosed. An example filtration device may include a funnel member having a first end region and a second end region. A flange may be coupled to the first end region. A filter support portion may be coupled to the second end region. The filter support portion may include a base, a filter membrane coupled to the base, and a securing member for securing the filter membrane to the base.

Abstract: A prosthetic heart valve provided herein can include a cultured cell tissue leaflet. In some cases, a prosthetic heart valve can include a plurality of leaflets secured together and retained within the expandable tubular member. The cultured cell tissue can be obtained by culturing fibroblast cells, smooth muscle cells, or a combination thereof to form a sheet of cultured cells and chemically cross-linking the fibroblast cells while under tension. In some cases, the cultured cell tissue can be radially tensioned. In some cases, the cultured cell tissue can be bi-axially tensioned.