Patents by Inventor Shannon Stott
Shannon Stott has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11612163Abstract: Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use.Type: GrantFiled: July 23, 2020Date of Patent: March 28, 2023Assignee: The General Hospital CorporationInventors: Rebecca Sandlin, Keith Wong, Shannon Tessier, Mehmet Toner, Shannon Stott
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Patent number: 11548002Abstract: Systems and techniques are described for capturing target extracellular vesicles from a fluid sample. In some implementations, a microfluidic device includes a microfluidic channel where an internal surface of at least one wall of the microfluidic channel includes a plurality of grooves or ridges, or both grooves and ridges, arranged and configured to generate chaotic mixing within a fluid sample flowing through the microfluidic channel. The microfluidic device also includes a plurality of elongate flexible linker molecules, each having a molecular weight between about 1.8-4.8 kDa, where each elongate flexible linker molecule is bound at a first end to an internal surface of at least one wall of the microfluidic channel and is bound at a second end to one or more binding moieties that specifically bind to a target extracellular vesicle.Type: GrantFiled: May 21, 2018Date of Patent: January 10, 2023Assignee: The General Hospital CorporationInventors: Eduardo Reategui, Shannon Stott, Mehmet Toner
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Publication number: 20220104482Abstract: Compositions, methods and kits are described for stabilizing cells and biological samples, allowing for storage and transportation of stabilized cells and biological samples. Cells can be stabilized in whole blood or fractionated blood. Isolated cells can also be stabilized.Type: ApplicationFiled: February 7, 2020Publication date: April 7, 2022Inventors: Mehmet Toner, Rebecca Sandlin, Anne Knecht, Ho Ki Wong, Shannon N. Tessier, Shannon Stott
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Publication number: 20210007348Abstract: Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use.Type: ApplicationFiled: July 23, 2020Publication date: January 14, 2021Inventors: Rebecca Sandlin, Keith Wong, Shannon Tessier, Mehmet Toner, Shannon Stott
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Patent number: 10750739Abstract: Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use. Exemplary applications include but are not limited to enrichment of leukocyte subtypes such as T-cells or neutrophils for cytokine and immuno-assays; isolation of progenitor cells from cord blood or peripheral blood for transplantation; isolation of fetal cells from the maternal blood for diagnosis; and sorting of circulating tumor cells for cancer detection and therapy.Type: GrantFiled: July 31, 2015Date of Patent: August 25, 2020Assignee: The General Hospital CorporationInventors: Rebecca Sandlin, Keith Wong, Shannon Tessier, Mehmet Toner, Shannon Stott
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Publication number: 20200122146Abstract: Methods and systems for isolating platelet-associated nucleated target cells, e.g., such as circulating epithelial cells, circulating tumor cells (CTCs), circulating endothelial cells (CECs), circulating stem cells (CSCs), neutrophils, and macrophages, from sample fluids, e.g., biological fluids, such as blood, bone marrow, plural effusions, and ascites fluid, are described. The methods include obtaining a cell capture chamber including a plurality of binding moieties bound to one or more walls of the chamber, wherein the binding moieties specifically bind to platelets; flowing the sample fluid through the cell capture chamber under conditions that allow the binding moieties to bind to any platelet-associated nucleated target cells in the sample to form complexes; and separating and collecting platelet-associated nucleated target cells from the complexes.Type: ApplicationFiled: August 22, 2019Publication date: April 23, 2020Inventors: Mehmet Toner, Shannon Stott, Eduardo Reategui, Xiaocheng Jiang
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Publication number: 20200070168Abstract: Systems and techniques are described for capturing target extracellular vesicles from a fluid sample. In some implementations, a microfluidic device includes a microfluidic channel where an internal surface of at least one wall of the microfluidic channel includes a plurality of grooves or ridges, or both grooves and ridges, arranged and configured to generate chaotic mixing within a fluid sample flowing through the microfluidic channel. The microfluidic device also includes a plurality of elongate flexible linker molecules, each having a molecular weight between about 1.8-4.8 kDa, where each elongate flexible linker molecule is bound at a first end to an internal surface of at least one wall of the microfluidic channel and is bound at a second end to one or more binding moieties that specifically bind to a target extracellular vesicle.Type: ApplicationFiled: May 21, 2018Publication date: March 5, 2020Inventors: Eduardo Reategui, Shannon Stott, Mehmet Toner
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Patent number: 10551376Abstract: Systems, methods, and devices for selective capture and release of target particles, e.g., living cells, from liquid samples, e.g., blood, are provided. The particle capture systems include a substrate; a first layer of gelatin bound to the substrate by physical adsorption, wherein the gelatin is functionalized with a plurality of first members of a binding pair; a second layer of gelatin wherein the gelatin is functionalized with a plurality of the first members of the binding pair and the second layer is bound to the first layer via a plurality of second members of the binding pair that are associated with the first members of the binding pair on both the first and the second layers; and a plurality of nanostructures bound to the second members of the binding pair and to one or more particle-binding moieties that selectively bind to the target particles.Type: GrantFiled: February 3, 2014Date of Patent: February 4, 2020Assignee: The General Hospital CorporationInventors: Eduardo Reategui, Shannon Stott, Mehmet Toner
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Patent number: 10391491Abstract: Methods and systems for isolating platelet-associated nucleated target cells, e.g., such as circulating epithelial cells, circulating tumor cells (CTCs), circulating endothelial cells (CECs), circulating stem cells (CSCs), neutrophils, and macrophages, from sample fluids, e.g., biological fluids, such as blood, bone marrow, plural effusions, and ascites fluid, are described. The methods include obtaining a cell capture chamber including a plurality of binding moieties bound to one or more walls of the chamber, wherein the binding moieties specifically bind to platelets; flowing the sample fluid through the cell capture chamber under conditions that allow the binding moieties to bind to any platelet-associated nucleated target cells in the sample to form complexes; and separating and collecting platelet-associated nucleated target cells from the complexes.Type: GrantFiled: August 7, 2015Date of Patent: August 27, 2019Assignee: The General Hospital CorporationInventors: Mehmet Toner, Shannon Stott, Eduardo Reategui, Xiaocheng Jiang
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Publication number: 20190072465Abstract: Methods and systems capturing particles suspended in a fluid flowed through a micro-channel, can include flowing the fluid including the particles to be captured through a micro-channel and past a groove defined in a surface of a wall of the micro-channel such that flowing the fluid past the groove forms microvortices in the fluid; contacting at least some of the particles against an adherent disposed on one or more of walls of the microchannel after the microvortices form in the fluid; and capturing at least some of the particles contacting the adherent.Type: ApplicationFiled: November 5, 2018Publication date: March 7, 2019Inventors: Mehmet Toner, Shannon Stott, Chia-Hsien Hsu
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Patent number: 10126218Abstract: Methods and systems capturing particles suspended in a fluid flowed through a micro-channel, can include flowing the fluid including the particles to be captured through a micro-channel and past a groove defined in a surface of a wall of the micro-channel such that flowing the fluid past the groove forms microvortices in the fluid; contacting at least some of the particles against an adherent disposed on one or more of walls of the microchannel after the microvortices form in the fluid; and capturing at least some of the particles contacting the adherent.Type: GrantFiled: September 2, 2015Date of Patent: November 13, 2018Assignee: The General Hospital CorporationInventors: Mehmet Toner, Shannon Stott, Chia-Hsien Hsu
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Patent number: 10018632Abstract: Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g.Type: GrantFiled: April 10, 2015Date of Patent: July 10, 2018Assignee: The General Hospital CorporationInventors: Thomas A. Barber, Ajay Shah, John Walsh, Mehmet Toner, Ravi Kapur, Shannon Stott
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Publication number: 20170225166Abstract: Methods and systems for isolating platelet-associated nucleated target cells, e.g., such as circulating epithelial cells, circulating tumor cells (CTCs), circulating endothelial cells (CECs), circulating stem cells (CSCs), neutrophils, and macrophages, from sample fluids, e.g., biological fluids, such as blood, bone marrow, plural effusions, and ascites fluid, are described. The methods include obtaining a cell capture chamber including a plurality of binding moieties bound to one or more walls of the chamber, wherein the binding moieties specifically bind to platelets; flowing the sample fluid through the cell capture chamber under conditions that allow the binding moieties to bind to any platelet-associated nucleated target cells in the sample to form complexes; and separating and collecting platelet-associated nucleated target cells from the complexes.Type: ApplicationFiled: August 7, 2015Publication date: August 10, 2017Inventors: Mehmet Toner, Shannon Stott, Eduardo Reategui, Xiaocheng Jiang
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Publication number: 20150377753Abstract: Methods and systems capturing particles suspended in a fluid flowed through a micro-channel, can include flowing the fluid including the particles to be captured through a micro-channel and past a groove defined in a surface of a wall of the micro-channel such that flowing the fluid past the groove forms microvortices in the fluid; contacting at least some of the particles against an adherent disposed on one or more of walls of the microchannel after the microvortices form in the fluid; and capturing at least some of the particles contacting the adherent.Type: ApplicationFiled: September 2, 2015Publication date: December 31, 2015Inventors: Mehmet Toner, Shannon Stott, Chia-Hsien Hsu
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Publication number: 20150369804Abstract: Systems, methods, and devices for selective capture and release of target particles, e.g., living cells, from liquid samples, e.g., blood, are provided. The particle capture systems include a substrate; a first layer of gelatin bound to the substrate by physical adsorption, wherein the gelatin is functionalized with a plurality of first members of a binding pair; a second layer of gelatin wherein the gelatin is functionalized with a plurality of the first members of the binding pair and the second layer is bound to the first layer via a plurality of second members of the binding pair that are associated with the first members of the binding pair on both the first and the second layers; and a plurality of nanostructures bound to the second members of the binding pair and to one or more particle-binding moieties that selectively bind to the target particles.Type: ApplicationFiled: February 3, 2014Publication date: December 24, 2015Inventors: Eduardo Reategui, Shannon Stott, Mehmet Toner
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Publication number: 20150285809Abstract: Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g.Type: ApplicationFiled: April 10, 2015Publication date: October 8, 2015Inventors: Thomas A. Barber, Ajay Shah, John Walsh, Mehmet Toner, Ravi Kapur, Shannon Stott
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Patent number: 9128091Abstract: Methods and systems capturing particles suspended in a fluid flowed through a micro-channel, can include flowing the fluid including the particles to be captured through a micro-channel and past a groove defined in a surface of a wall of the micro-channel such that flowing the fluid past the groove forms microvortices in the fluid; contacting at least some of the particles against an adherent disposed on one or more of walls of the microchannel after the microvortices form in the fluid; and capturing at least some of the particles contacting the adherent.Type: GrantFiled: September 25, 2009Date of Patent: September 8, 2015Assignee: The General Hospital CorporationInventors: Mehmet Toner, Shannon Stott, Chia-Hsien Hsu