Abstract: A method includes steps of: performing preparation-phase measurements on a spindle of a machine tool to generate normal-condition signals; establishing a reference model based on the normal-condition signals; generating abnormal-condition signals based on the reference model and a preset damage value; utilizing principal components analysis (PCA) to characterize the normal-condition and abnormal-condition signals to obtain normal and abnormal probabilistic models, determining normal-condition and abnormal-condition reference curves based on the normal and abnormal probabilistic models; determining an alert-triggering line based on the abnormal-condition reference curve; determining a permissible range between the alert-triggering line and the normal-condition reference curve; and generating a warning signal when it is determined that a detection value falls outside of the permissible range, wherein the detection value is obtained based on application-phase measurements performed on the spindle.

Abstract: A manufacturing method of separating and purifying neoagarooligosaccharides having degrees of polymerization of 2-22 includes the steps of adding crude enzyme solutions with agarases produced by Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108, respectively, into an agar polysaccharide extract solution, and obtaining a neoagarooligosaccharides solution after a hydrolysis on the algal polysaccharide (such as agar) is performed; performing a separation to the neoagarooligosaccharides solution by a ultrafiltration (UF) system to obtain a neoagarooligosaccharide eluent; performing a separation to the neoagarooligosaccharide eluent by a semi-preparative high-performance liquid chromatography system equipped with a molecule size exclusion chromatography function to obtain the neoagarooligosaccharides having the degrees of polymerization from 2 to 22; and using a fraction collector to collect the neoagarooligosaccharides, and obtaining purified single neoagarooligosaccharide products with different mo