Abstract: An immune molecule virus particle detection kit is provided. A monoclonal antibody of a virus envelope antigen is modified with biotin, magnetic beads are coupled with streptavidin, and the biotin-modified monoclonal antibody is incubated with a virus-containing solution to form a complex with virus particles or antigens, and then the streptavidin-coupled magnetic beads are added for incubation. The streptavidin on the magnetic beads binds with the biotin-modified monoclonal antibody with high specificity and affinity, and then specifically captures the virus particles with envelopes. After separating a supernatant through a magnetic separator, complete viral particles, empty-shell viruses, and free envelope antigens can be separated from other virus components, and the magnetic bead conjugates can be qualitatively or quantitatively detected through polymerase chain reaction (PCR) amplification.