Abstract: The present invention relates to a kit for detecting a subset of a human regulatory T cell, and a detection method thereof, and belongs to the technical field of cell subset detection. The present invention provides a kit which includes the following components: a blood dilution solution, a mononuclear cell isolation solution, a cell culture solution, a lymphocyte activation liquid, a dead-cell-removing dye, an FcR blocking agent, fluorescence-labeled antibodies against human cell-surface markers, fluorescence-labeled antibodies against human intracellular molecules, a PBS buffer, lipopolysaccharide, a washing buffer, a cell staining buffer, a cell fixing solution, and a membrane-permeable wash buffer. At the time of detection, firstly mononuclear cells are isolated from whole blood, secondly human peripheral blood mononuclear cells are stimulated, and finally the subsets of the regulatory T cells can be detected just by fluorescent antibody staining.

Abstract: A freely-assembled ELISA plate is provided, including a first ELISA plate frame, a second ELISA plate frame, and ELISA plate strips located between the first ELISA plate frame and the second ELISA plate frame, collectively forming a liquid flow groove. The first ELISA plate frame is connected to a first side of the ELISA plate strips. A second side of the ELISA strips is connected to the second ELISA plate frame. The number of the ELISA plate strips in the ELISA plate can be determined according to the sample size, so that the waste of well resources of the ELISA plate can be effectively reduced; and, a desired amount of samples, washing buffer or the like can be added into the liquid flow groove once for all, so that the sample loading amount can be reduced and the detection speed can be quickened.

Abstract: A freely-assembled ELISA plate is provided, including a first ELISA plate frame, a second ELISA plate frame, and ELISA plate strips located between the first ELISA plate frame and the second ELISA plate frame, collectively forming a liquid flow groove. The first ELISA plate frame is connected to a first side of the ELISA plate strips. A second side of the ELISA strips is connected to the second ELISA plate frame. The number of the ELISA plate strips in the ELISA plate can be determined according to the sample size, so that the waste of well resources of the ELISA plate can be effectively reduced; and, a desired amount of samples, washing buffer or the like can be added into the liquid flow groove once for all, so that the sample loading amount can be reduced and the detection speed can be quickened.

Abstract: An ELISA plate is provided, and includes several ELISA plate wells, each of which includes a hemispherical bottom, a funnel-shaped formation arranged in the middle and connected to the hemispherical bottom, and a projecting wall arranged in an upper part and connected to the funnel-shaped formation. The hemispherical bottom of the ELISA plate can increase the surface area of the ELISA plate over conventional designs, thereby increasing the opportunity of contact between a target substance and a target molecule on the surface of the solid phase and enabling detection of the content of the target substance using less sample amount. The funnel-shaped formation of the ELISA plate well is arranged to be connected to the bottom and upper part of the ELISA plate well, thereby further increasing the surface area of the ELISA plate well.