Patents by Inventor Shaohong CHANG

Shaohong CHANG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for preparing bacterial polysaccharide-modified recombinant fusion protein and use of the protein
    Patent number: 10844098
    Abstract: The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and the Neisseria meningitidis O-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: November 24, 2020
    Assignee: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun Wu, Chao Pan, Peng Sun, Hengliang Wang, Bo Liu, Zhehui Peng, Li Zhu, Shaohong Chang, Xin Gong, Erling Feng, Bin Wang, Ming Zeng
  • with an O-antigen having an extended carbohydrate chain and use thereof
    Patent number: 10780154
    Abstract: The present invention discloses a Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.
    Type: Grant
    Filed: September 7, 2015
    Date of Patent: September 22, 2020
    Assignee: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun Wu, Hengliang Wang, Peng Sun, Bo Liu, Li Zhu, Chao Pan, Tiantian Wang, Xin Gong, Shaohong Chang, Erling Feng
  • with an O-antigen having an extended carbohydrate chain and use thereof
    Patent number: 10709777
    Abstract: The present invention discloses a Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.
    Type: Grant
    Filed: September 7, 2015
    Date of Patent: July 14, 2020
    Assignee: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun Wu, Peng Sun, Bo Liu, Shaohong Chang, Xin Gong, Hengliang Wang, Li Zhu, Chao Pan, Erling Feng
  • Method for Preparing Bacterial Polysaccharide-Modified Recombinant Fusion Protein and Use of the Protein
    Publication number: 20180258145
    Abstract: The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and the Neisseria meningitidis O-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.
    Type: Application
    Filed: September 1, 2015
    Publication date: September 13, 2018
    Applicant: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun WU, Chao PAN, Peng SUN, Hengliang WANG, Bo LIU, Zhehui PENG, Li ZHU, Shaohong CHANG, Xin GONG, Erling FENG, Bin WANG, Ming ZENG
  • A Salmonella Paratyphi A with an O-Antigen Having an Extended Carbohydrate Chain and Use Thereof
    Publication number: 20180050100
    Abstract: The present invention discloses a Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.
    Type: Application
    Filed: September 7, 2015
    Publication date: February 22, 2018
    Applicant: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun WU, Peng SUN, Bo LIU, Shaohong CHANG, Xin GONG, Hengliang WANG, Li ZHU, Chao PAN, Erling FENG