Abstract: The present application provides a method for preparing a prelithiated positive electrode current collector, the method comprising the following steps: gasifying a lithium source in an evaporation chamber filled with a protective gas; enabling an aluminum foil layer to pass through the evaporation chamber and depositing the metal lithium on a surface of the aluminum foil layer by means of vapor deposition; and transferring the aluminum foil layer deposited with the metal lithium to an annealing chamber filled with a protective gas and performing high-temperature annealing to form a lithium-aluminum alloy layer on the surface of the aluminum foil layer.

Abstract: The present disclosure discloses three carotenoid esterases and the application of their encoding genes. The genes encoding the carotenoid esterase include: LbZAT1 gene with the nucleotide sequence as shown in SEQ ID NO.1, LbZAT2 gene with the nucleotide sequence as shown in SEQ ID NO.2, and/or LbZAT3 gene with the nucleotide sequence as shown in SEQ ID NO.3. The proteins encoded by the LbZAT1, LbZAT2 and LbZAT3 genes of the present disclosure have the function of catalyzing the esterification reaction between carotenoids containing free hydroxyl and fatty acid acyl donors. Carotenoid esterase and its encoding genes have important application value in the biosynthesis of esterified carotenoids in vitro.