Abstract: The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.

Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid (SDA) and fermentation technology. ?15 Desaturase gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and ?15 Desaturase gene is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-?15, finally, the expression of the ?15 Desaturase gene in Mucor circinelloides is realized. The recombinant new strain is accession number CGMCC No. 15888, and the classification name is Mucor circinelloides-D15D.

Abstract: The present application discloses a dicarboxylic acid transporter and its encoding gene dit gene for increasing oil yield of Mucor circinelloides, the dit gene is cloned from the high-yield M. circinelloides WJ11, and the dit gene is transformed into M. circinelloides deficient strain Mu402, the dit gene is integrated into the genome of M. circinelloides by homologous recombination to obtain recombinant strain Mc-Dit. The total fatty acid content of the Mc-Dit strain increased by 33.76% and the intracellular lipid content may reach up to 17.67% of the dry biomass.

Abstract: The present disclosure provides a natural plant preparation, including extracts of the following parts of plants: roots of Salvia miltiorrhiza Bunge, Juglans regia, Aesculus hippocastanum L. and Zingiber officinale Rosc., barks of Cinnamomum cassia Presl, seeds of Sesamum indicum, Carica papaya L., Nigella damascena L. and Punica granatum L., leaves of Centaurea cyanus L., resin of Pistacia Lentiscus, drupes of Cocos nucifera L., beans of Theobroma cacao L., dried pulp and kernels of Phoenix dactylifera L., dried pulp of Olea europaea, Ficus carica Linn., Walnut and Vaccinium Spp, cloves of Allium sativum, and petals of Rosa sp. The present disclosure further provides a method for preparing a plant preparation, including treating the above parts of plants with ethanol.

Abstract: Provided is construction of Mucor circinelloides cell factory for producing stearidonic acid SDA and fermentation technology thereof. ?15 Des gene is obtained by cloning from Mortierella alpina, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and ?15 Des is integrated on Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-?15, finally, the expression of the ?15 Des gene in Mucor circinelloides is realized. The recombinant new strain was preserved in China General Microbiological Culture Collection Center on Jun. 20, 2018, and the address is NO. 1 West Beichen Road, Chaoyang District, Beijing. The center gave the biological material the preservation number CGMCC No. 15888, and the suggested classification name is Mucor circinelloides-D15D.

Abstract: The present disclosure provides a natural plant preparation, including extracts of the following parts of plants: roots of Salvia miltiorrhiza Bunge, Juglans regia, Aesculus hippocastanum L. and Zingiber officinale Rosc., barks of Cinnamomum cassia Presl, seeds of Sesamum indicum, Carica papaya L., Nigella damascena L. and Punica granatum L., leaves of Centaurea cyanus L., resin of Pistacia Lentiscus, drupes of Cocos nucifera L., beans of Theobroma cacao L., dried pulp and kernels of Phoenix dactylifera L., dried pulp of Olea europaea, Ficus carica Linn., Walnut and Vaccinium Spp, cloves of Allium sativum, and petals of Rosa sp. The present disclosure further provides a method for preparing a plant preparation, including treating the above parts of plants with ethanol.