Abstract: The invention discloses a protective device for use during autopsy by a prosector operating with handheld instruments. The autopsy subject is covered by a transparent dissection chamber-forming device which is attached to the draped about the body of the subject. By attaching the device to the body of the subject the dissection site is isolated from the prosector and his support staff. The device provides an instrument port through which the handheld instrument can be partially inserted and further the device provides for sealing against the instrument. In addition to the device, a method of protecting autopsy room staff is also disclosed. Through the device and the associated method, the prosector is enabled to use, with an unobstructed view of the autopsy subject, handheld instruments while the effluent from the dissection of the autopsy subject is restricted within the sealed dissection chamber. The autopsy room staff is thus protected from infection.