Abstract: A seed crusher e.g. useful in methods for high throughput analysis of seeds, comprises a horizontal well plate with at least one well for receiving a tube; an open-topped tube for lining each well wherein each tube is oriented for receiving a vertical reciprocating pin; a horizontal die plate with a vertical pin located to reciprocate in each tube; a removable separator for use in each tube to separate a reciprocating pin from a seed at the bottom of said tube and a press for bringing together the die plate and well plate to allow a pin to enter each tube.

Abstract: Methods of genotyping amplified mixtures of DNAs, nucleic acid markers and methods of obtaining markers, kits, recombinant plants, positional cloning and integrated systems for making genotypes and assessing hybridizations are provided. These features are applicable to DNA fingerprinting, marker assisted selection, genotyping, cladistic analysis of variance, and high throughput laboratory screening methods.