Abstract: A process isolates and recovers glycoprotein fractions in dry or solution form. Glycoproteins are recovered from deproteinized whey, preferably micro-filtered to remove large molecules and aggregates. The resulting retentate is then diluted for further processing. The resulting liquid is heated to coagulate whey protein and then cooled sufficiently to precipitate coagulated whey protein. The preparation can then be completed by centrifuging the resulting cooled solution and separating resulting supernatant containing glycoproteins from fat and precipitate. The product glycoprotein concentrate can be dried, such as by freeze drying, or recovered and stored in liquid form. In a preferred aspect, saline is employed to dilute the microfiltered concentrate prior to heating to improve the recovery of a liquid glycoprotein fraction that can be sterilized, such as by autoclaving.

Abstract: A Process is provided for preparing MUC1 from bovine milk, whey or deproteinized whey. In one aspect the process involves providing concentrated whey derived from bovine milk; acidifying the whey; separating soluble whey protein from the resulting acidified whey to leave a MUC1-containing fraction containing insoluble membrane protein and low pI protein; and then separating MUC1 from the MUC1-containing fraction. In another, aspect the invention involves treating deproteinized whey derived from bovine milk with a whey protein solubilizing solution; centrifuging the treated whey to recover a soluble supernatant fraction; adsorbing protein on a buffer-washed PNA; separating the PNA from remaining liquid; and eluting MUC1 from said PNA. The MUC1 is pure and can be freeze dried.

Abstract: A process isolates and recovers glycoprotein fractions in dry or solution form. Glycoproteins are recovered from deproteinized whey, preferably micro-filtered to remove large molecules and aggregates. The resulting retentate is then diluted for further processing. The resulting liquid is heated to coagulate whey protein and then cooled sufficiently to precipitate coagulated whey protein. The preparation can then be completed by centrifuging the resulting cooled solution and separating resulting supernatant containing glycoproteins from fat and precipitate. The product glycoprotein concentrate can be dried, such as by freeze drying, or recovered and stored in liquid form. In a preferred aspect, saline is employed to dilute the microfiltered concentrate prior to heating to improve the recovery of a liquid glycoprotein fraction that can be sterilized, such as by autoclaving.

Abstract: The production of GMP in suitable quantities and of suitable quality for supply to the food, pharmaceutical, cosmetic, and other industries, is provided. The overall cheese making is made more efficient by recovering valuable kappa-casein glycomacropeptides from whey in a manner that permits most whey protein to be separated from the whey prior to concentrating and recovering glycomacropeptides from bovine whey. The invention provides procedures working on concentrated micro-filtered deproteinized whey protein (MFDPW) and obtaining a purified residue which can be dried.