Abstract: The present invention provides an antibody binding a human tumor-associated calcium signal sensor 2 (Trop-2) protein or fragments thereof, and use of the antibody or fragments thereof in preventing or treating diseases. The antibody or fragments thereof of the present invention can effectively bind to the human Trop-2 protein, and have internalization activity, and the internalization activity is enhanced after ADC drug labeling, and the in vivo efficacy and safety of a mouse model are not lower than those of a control antibody.

Abstract: A variable region sequence of a specific antibody against clothianidin is provided. A gene encoding a heavy chain variable region of the antibody has an amino acid sequence shown in SEQ ID NO: 2. An intact recombinant antibody against clothianidin is provided. The intact recombinant antibody includes a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, wherein a gene encoding the heavy chain variable region has an amino acid sequence shown in SEQ ID NO: 2. An immunostrip containing the antibody for rapid detection of clothianidin residue is also provided. The sequence genes obtained are linked to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, respectively, and an intact recombinant antibody is expressed and obtained by using mammalian cells with a double-plasmid system.

Abstract: Provided are an antibody and a preparation method therefor. The antibody is, such as a humanized anti-human CD47 monoclonal antibody or fragment thereof, and a bispecific antibody targeting PD-L1 and CD47 or an antigen-binding fragment thereof. The bispecific antibody comprises two heavy chains and two light chains, variable regions of the two heavy chains are heterologous, and variable regions of the two light chains have similar sequences. The provided bispecific antibody has the light chains having the similar sequences, thus solving the technical problem of light and heavy chain mismatch during an assembly process of bispecific antibodies.

Abstract: In view of the technical problem that TGF-? RH and a fusion protein thereof are prone to degradation and breakage during the process of recombinant expression, provided are a TGF-? RII mutant and a fusion protein thereof. Compared with the extracellular region of the wild-type TGF-? RII of SEQ ID NO: 6, the TGF-? RII mutant has a mutation selected from Gln at position 6, Asp at position 12 and Gly at position 20. The TGF-? RII mutant is capable of binding to TGF-?. Compared to wild-type TGF-? RII, the TGF-? RII mutant has less breakage and/or degradation during recombination expression. It is convenient for large-scale production of antibodies/the TGF-? RII bifunctional protein with a more stable quality.

Abstract: Provided are a single variable domain antibody targeting a human programmed death ligand 1 (PD-L1) and a derivative thereof. A single variable domain antibody 2-2F2 specific for human PD-L1 is identified and obtained by extracting PBMCs from a camel immunized with human PD-L1, constructing a phage surface displayed VHH antibody library, and screening said VHH antibody library. On said basis, a chimeric antibody chF2 and a humanized modified antibody hzF2 variant are prepared. The hzF2 variant possesses affinity comparable or even superior to that of the original single variable domain antibody 2-2F2, can block the binding of PD-1 to PD-L1 in vitro, and can inhibit tumor growth in an in vivo experiment on tumor-bearing mice.

Abstract: A variable region sequence of a specific antibody against clothianidin is provided. A gene encoding a heavy chain variable region of the antibody has an amino acid sequence shown in SEQ ID NO: 2. An intact recombinant antibody against clothianidin is provided. The intact recombinant antibody includes a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, wherein a gene encoding the heavy chain variable region has an amino acid sequence shown in SEQ ID NO: 2. An immunostrip containing the antibody for rapid detection of clothianidin residue is also provided. The sequence genes obtained are linked to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, respectively, and an intact recombinant antibody is expressed and obtained by using mammalian cells with a double-plasmid system.

Abstract: The present invention provides an antibody binding to programmed cell death protein 1 (PD-1) or a fragment thereof and a use of the antibody or a fragment thereof in preventing or treating a tumor or cancer. The antibody or a fragment thereof of the present invention can effectively block the interactions of PD-1/PD-L1 and PD-1/PD-L2 and has a unique antigen binding epitope and unique properties in terms of efficacy and safety.

Abstract: A cell structure of a silicon carbide MOSFET device, comprising a drift region (3) located on a substrate layer (2), a second conducting type well region (4) and a first JFET region (51) that are located in the drift region (3), an enhancement region located within a surface of the well region (4), a gate insulating layer (8) located on a first conducting type enhancement region (6), the well region (4) and the first JFET region (51) and being in contact therewith at the same time, a gate (9) located on the gate insulating layer, source metal (10) located on the enhancement region, Schottky metal (11) located on a second conducting type enhancement region (7) and the drift region (3), a second JFET region (52) located on a surface of the drift region (3) between the Schottky metals (11), and drain metal (12).