Abstract: Programmable guide RNAs (gRNAs) play a central role in the CRISPR revolution sweeping biology and medicine by directing the function of a Cas protein effector to a target gene of choice. To achieve programmable control over regulatory scope, the activity of a conditional guide RNA (cgRNA) depends on the presence or absence of an RNA trigger, allowing for cell-selective regulation of CRISPR/Cas function. Unlike a standard gRNA, a cgRNA is programmable at multiple levels, with the target-binding sequence controlling the target of Cas activity (edit, silence, induce, or bind a gene of choice) and the triggerbinding sequence controlling the scope of Cas activity. cgRNA mechanisms that are allosteric allow for independent design of the target and trigger sequences, providing the flexibility to select the regulatory target and scope independently.

Abstract: Some embodiments provide a novel method for assessing the suitability of network links for connecting compute nodes located at different geographic sites. The method of some embodiments identifies and analyzes sample packets from a set of flows exchanged between first and second compute sites that are connected through a first network link in order to identify attributes of the sampled packets. The method also computes attributes of predicted packets between the identified samples in order to identify attributes of each flow in the set of flows. The method then uses the identified and computed attributes of each flow in the set of flows to emulate the set of flows passing between the two compute sites through the second network link in order to assess whether a second network link should be used for future flows (e.g., future flows exchanged between the first and second compute sites).

Abstract: Programmable guide RNAs (gRNAs) play a central role in the CRISPR revolution sweeping biology and medicine by directing the function of a Cas protein effector to a target gene of choice. To achieve programmable control over regulatory scope, the activity of a conditional guide RNA (cgRNA) depends on the presence or absence of an RNA trigger, allowing for cell-selective regulation of CRISPR/Cas function. Unlike a standard gRNA, a cgRNA is programmable at multiple levels, with the target-binding sequence controlling the target of Cas activity (edit, silence, induce, or bind a gene of choice) and the trigger binding sequence controlling the scope of Cas activity. cgRNA mechanisms that are allosteric allow for independent design of the target and trigger sequences, providing the flexibility to select the regulatory target and scope independently.

Abstract: Programmable guide RNAs (gRNAs) play a central role in the CRISPR revolution sweeping biology and medicine by directing the function of a Cas protein effector to a target gene of choice. To achieve programmable control over regulatory scope, the activity of a conditional guide RNA (cgRNA) depends on the presence or absence of an RNA trigger, allowing for cell-selective regulation of CRISPR/Cas function. Unlike a standard gRNA, a cgRNA is programmable at multiple levels, with the target-binding sequence controlling the target of Cas activity (edit, silence, induce, or bind a gene of choice) and the trigger binding sequence controlling the scope of Cas activity. cgRNA mechanisms that are allosteric allow for independent design of the target and trigger sequences, providing the flexibility to select the regulatory target and scope independently.

Abstract: Methods and systems for generating a diagram from structured data and synchronizing modifications between the diagram and the structured data. One system includes at least one processor configured to receive a plurality of transformation settings for the structured data and generate and store a plurality of first expressions and a plurality of second expressions based on the structured data and the plurality of transformation settings. The processor is further configured to generate and organize a plurality of visual structures based on the plurality of first expressions and the plurality of second expressions and generate and output a diagram for display through a user interface including the plurality of visual structures. In addition, the processor is configured to receive a modification to the diagram through the user interface, and modify the structured data based on the modification, the plurality of first expressions, and the plurality of second expressions.

Abstract: Methods and systems for generating a diagram from structured data and synchronizing modifications between the diagram and the structured data. One system includes at least one processor configured to receive a plurality of transformation settings for the structured data and generate and store a plurality of first expressions and a plurality of second expressions based on the structured data and the plurality of transformation settings. The processor is further configured to generate and organize a plurality of visual structures based on the plurality of first expressions and the plurality of second expressions and generate and output a diagram for display through a user interface including the plurality of visual structures. In addition, the processor is configured to receive a modification to the diagram through the user interface, and modify the structured data based on the modification, the plurality of first expressions, and the plurality of second expressions.