Abstract: Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.

Abstract: A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation.

Abstract: A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.

Abstract: Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir.

Abstract: Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir.

Abstract: Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.

Abstract: Methods and microfluidic devices for generating and manipulating sample droplets, wherein the devices comprise, a plurality of fluid channels, at least one of which is a sample channel for carrying a fluidic sample material, that is in fluid communication with the carrier fluid channel via an orifice; and an actuated flow interrupter adapted to force a predetermined amount of the sample fluid from the sample channel through the orifice into the carrier fluid channel.

Abstract: A microfluidic chip comprising a separation channel configured to receive a sieving matrix and a buffer and an injection channel in fluid communication with the separation channel. The injection channel is configured to receive a sample using a capillary force and a portion of the sample injects into the separation channel electro-kinetic force exerted on the sample.

Abstract: A microchip for electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well. The injection channel and the separation channel form a ‘T’ junction. The microchip comprises a first electrode disposed at a first end of the separation channel, a second electrode disposed in front of the ‘T’ junction and adjacent to the first electrode, a third electrode disposed at a first end of the injection channel and a fourth electrode disposed at a second end of the separation channel. A portion of the sample is injected and separated into an area between the ‘T’ junction and the fourth electrode.

Abstract: A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.

Abstract: A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation.

Abstract: A microsampler disc for use in the analysis of agents is described as including a plurality of microstructures configured and spaced to promote movement of a fluidic medium containing agents radially outwardly and promote filtering of one species of agents from other species of agents. An analysis system using the microsampler disc is also described. A method for separating one species of agent from one or more other species of agents is described as including introducing a fluidic medium containing at least one species of agents to a microsampler disc having a plurality of microstructures, rotating the microsampler disc to promote movement of the fluidic medium outwardly, collecting the at least one species of agent in a specific set of detection zones, and analyzing the at least one species of agent.