Abstract: High-throughput methods for mapping integration sites resulting from one or more integrations, such as infection by a retrovirus, are disclosed. The disclosed methods require no selection for specific phenotypes such as antibiotic resistance, and thereby may avoid selection bias. Moreover, the linker-based amplification is simple and rapid, and by using a frequently cutting restriction enzyme, the amplicons are small, which significantly decreases possible amplification and cloning biases.

Abstract: The present invention provides cells comprising an isolated nucleic acid encoding a novel lactate dehydrogenase (LDH) and/or the encoded LDH polypeptide. The invention further provides cells comprising an isolated nucleic acid encoding LDH, wherein the cell is capable of producing and secreting insulin. Also provided are methods of providing fuel-stimulated (e.g., glucose-stimulated) insulin secreting capability to a mammalian subject by implanting cells of the invention (e.g., in a semi-permeable membrane and/or an implantable device) into the subject. Further provided are devices comprising the cells of the invention. In particular embodiments, the device is an implantable device.

Abstract: The present invention provides an isolated nucleic acid encoding a novel lactate dehydrogenase (LDH) as well as the isolated LDH polypeptide. Also provided are methods of enhancing fuel-stimulated insulin secretion, in particular, glucose-stimulated insulin secretion. Further provided are methods of screening for compounds that bind LDH, modulate LDH activity, and/or modulate fuel- or glucose-stimulated insulin secretion and the compounds identified thereby. The invention further provides a method of enhancing insulin secretion by administering a nucleic acid encoding LDH to a subject in a therapeutically effective amount.