Patents by Inventor Shawn P. Cain

Shawn P. Cain has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Removal of agent from cell suspension
    Patent number: 6068775
    Abstract: The invention relates to a method of removing an agent from a suspension of cells using a semi-permeable membrane. In one aspect of the invention, the cells are used to bioprocess a biological fluid after removal of the agent.
    Type: Grant
    Filed: April 13, 1998
    Date of Patent: May 30, 2000
    Assignee: Circe Biomedical, Inc.
    Inventors: Linda M. Custer, Shawn P. Cain, Barbara A. Chandler
  • High flow technique for harvesting mammalian cells
    Patent number: 5912163
    Abstract: The invention is based in part on the observation that significant portions of porcine livers appear to remain intact after perfusion by standard methods, suggesting that the perfusion procedures employed do not result in complete enzymatic digestion. Recovery of cells is therefore substantially lower than would be possible if the organs were thoroughly digested. It is found that increased perfusion flow rate, occlusion of at least one major blood vessel leading out of the organ, increased enzymatic digestion time, and vigorous tissue dissociation techniques can be combined to afford a uniquely high yield of viable cells.
    Type: Grant
    Filed: October 20, 1997
    Date of Patent: June 15, 1999
    Assignee: Circe Biomedical, Inc.
    Inventors: Kermit M. Borland, Barbara A. Chandler, Dianna Hunt Picton, Shawn P. Cain, Deborah Deane, Claudy J.P. Mullon
  • Method of thawing cryopreserved cells
    Patent number: 5895745
    Abstract: The invention features a method of processing cryopreserved cells by thawing and equilibrating the cells at warm temperatures (e.g., between 30.degree. C. and 43.degree. C). Either the cell suspension in the cryoprotective medium is thawed to a temperature between 35.degree. C. and 43.degree. C. or the cryoprotective medium is equilibrated with a culture medium at a temperature between 35.degree. C. and 43.degree. C., or both steps are carried out at the warm temperatures. By thawing and equilibrating the cryopreserved cells at warm temperatures, the viability, (especially after 3 hours of culture), and metabolic activity (i.e., diazepam metabolism) of the cells can be improved over traditional cold cell processing (i.e., at temperatures of between 2.degree. C. and 8.degree. C.).
    Type: Grant
    Filed: September 25, 1996
    Date of Patent: April 20, 1999
    Assignee: W.R. Grace & Co.-Conn.
    Inventors: Barbara A. Chandler, Kermit M. Borland, Shawn P. Cain, Claudy J-P. Mullon
  • Closed system for processing cells
    Patent number: 5858642
    Abstract: The invention features a method of processing cells in a closed system that results in a suspension of cells in a transfer vessel containing a target number of cells. The number of cells in the closed vessel is determined from the cell concentration (i.e., the number of viable cells/mL) in the closed vessel and the total volume of the suspension in the closed vessel. The volume of the suspension in the closed vessel can be determined from the weight of the suspension and its density. In particular, the cells are preserved in a protective medium and are recovered substantially free of the protective medium in a closed vessel containing the known number of cells in a suspension.
    Type: Grant
    Filed: September 25, 1996
    Date of Patent: January 12, 1999
    Assignee: W.R. Grace & Co.-Conn.
    Inventors: Shawn P. Cain, Timothy J. Perlman, Deborah J. Deane, Claudy J-P. Mullon