Abstract: Provided herein are methods and compositions relating to at least partially recellularized human organs. Various methods of decellularizing non-human animal organs and recellularizing a non-human animal extracellular matrix with cell compositions. Further provided are compositions and methods for treating a liver or other disease (such as acute liver failure) using an extracorporeal bioengineered liver or other organ.

Abstract: A system (200) for growing or supporting an organ (50), in particular a biologically engineered organ, comprises: an enclosure (202); a perfusate circuit: a perfusate pump (206); a gas transfer unit (208) and a optionally a gas mixture unit connected to the gas transfer unit; one or more sensors (212a, 212b); an injection system (216) configured to inject nutrients; and a controller (214). The controller can be configured to operate the pump, the gas transfer unit, and the injection system based on sensor signals from the sensor(s) to grow or support the organ in an automated or semi-automated fashion.

Abstract: The disclosure provides for methods of sealing of decellularized and recellularized engineered organ grafts, thereby providing a reinforced (fortified) decellularized and recellularized engineered organ graft.