Abstract: Provided is a feed ingredient comprising propionate and a byproduct of fermentation of a feedstock by propionic acid-producing bacteria, wherein said feedstock comprises a carbon source and wherein said bacteria are of a class selected from the group consisting of Actinobacteria, Bacteroidia, Clostridia, Negativicutes and combinations thereof. Also provided are methods for manufacturing the feed ingredient and uses thereof.

Abstract: Provided is a method for the manufacture of a butyrate product by fermentation of a carbon source-containing fermentation feedstock with a Clostridia class bacterium which natively produces butyric acid while maintaining a pH of about 5 to 7, wherein ammonia is used for said maintaining pH and whereby a fermentation broth comprising ammonium butyrate, a biomass, and optionally a fermentation by-product is formed; separating said biomass from said fermentation broth to form separated biomass and a clarified fermentation broth, wherein said clarified fermentation broth comprises said ammonium butyrate and optionally said fermentation by-product; and adding to said clarified broth an additive selected from the group consisting of a mineral acid, a mineral base, a soluble calcium salt and combinations thereof, whereby said butyrate product is formed in said clarified broth. Further provided are feed ingredients, animal feeds and anti-icing agents comprising the composition, and uses thereof.

Abstract: Provided are compositions comprising at least one organism of a strain of the order Clostridiales and methods of use thereof for protecting against an infection, such as a viral, protozoal or fungal infection in an animal. Also provided are methods for improving hatchability of an egg under contaminated conditions by protecting against infection of an avian in ovo.

Abstract: Provided are microbial compositions comprising at least one attenuated form of a pathogenic bacterium having an ability to colonize at least one organ of the gastrointestinal tract of an animal, such as an attenuated form of a bacterium having an antagonistic effect against a bacterium selected from the group consisting of Salmonella enteritidis, Salmonella enterica, Salmonella typhimurium, lostridium difficile, Clostridium perfringens, Escherichia coli, Campylobacter spp., Listeria monocytogenes, Yersinia enterocolitica and combinations thereof. Also provided are animal feeds comprising such compositions, preparations for in-ovo injection comprising such compositions and methods of treatment using such compositions.

Abstract: Provided is a probiotic composition, comprising at least one genus of mixotrophic microorganism and a carrier and pharmaceutical preparations comprising such a composition for use in the treatment of bloating in an animal. Also provided are methods of use of such pharmaceutical preparations in the treatment of bloating in an animal.

Abstract: Supplemented mixotrophic method. A mixotrophic fermentation method is disclosed including providing a naturally acetogenic organism; providing a fermentation medium comprising a carbon source and a supplemented non-sugar reductant; and culturing the organism in the fermentation medium, where both the carbon source and the non-sugar reductant are metabolized and a fermentation broth is formed, which contains at least one carbon-containing bioproduct.

Abstract: Provided are methods and compositions an organism of the order Clostridiales for improving the health of an avian, wherein an effective dose of the composition is applied to an outer surface of an egg containing the avian as an embryo prior to hatching. Also disclosed are compositions comprising a strain within the order Clostridiales selected from the group consisting of Caldicoprobacteraceae; Christensenellaceae; Clostridiaceae; Defluviitaleaceae; Eubacteriaceae; Graciibacteraceae; Heliobacteriaceae; Lachnospiraceae; Oscillospiraceae; Peptococcaceae; Peptostreptococcaceae; Ruminococcaceae; Syntrophomonadaceae and combinations thereof and their use in such methods.

Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.

Abstract: A selection method including providing a microorganism preparation including a variety of microorganisms and optionally water; providing an organic liquid including at least 70% by weight hydrophobic solvent; forming a multiple phase medium including a selected amount of the microorganism preparation and a selected amount of the organic liquid; and maintaining the multiple phase medium at a selected temperature for selected duration, whereby a treated microorganism preparation is formed.

Abstract: Provided is an improved method for selecting a probiotic microorganism composition, the method comprising providing a first consortium of microorganisms having Microorganism Composition A; providing a first host animal having a first microbiome having Microorganism Composition B; administering said first consortium to said first host animal, whereby said first microbiome is converted to a second microbiome having Microorganism Composition C; collecting a first sample of said second microbiome; and transferring at least a fraction of said first sample and/or said treated first sample onto and/or into a growth medium and incubating at a temperature between 15 degrees Celsius and 70 degrees Celsius to form first selected microorganism composition having Microorganism Composition D; wherein Microorganism Composition A of said first consortium comprises at least 50 different strains; wherein each of Compositions A and B; B and C and D and A differ from each other.

Abstract: Provided is a feed ingredient comprising at least 20% by weight Clostridium tyrobutyricum, wherein said Clostridium tyrobutyricum comprises at least 70% of dry weight crude protein and optionally at least 7% of dry weight lysine and/or at least 1% of dry weight methionine. Further provided are animal feeds and probiotic compositions comprising the feed ingredient, methods for the production thereof and uses thereof.

Abstract: Supplemented mixotrophic method. A mixotrophic fermentation method is disclosed including providing a naturally acetogenic organism; providing a fermentation medium comprising a carbon source and a supplemented non-sugar reductant; and culturing the organism in the fermentation medium, where both the carbon source and the non-sugar reductant are metabolized and a fermentation broth is formed, which contains at least one carbon-containing bioproduct.

Abstract: An integrated wet-mill method for the production of ethanol and single cell protein. An integrated method for the production of ethanol and a single cell protein product is described including providing corn kernels, which kernels comprise germ, fiber, protein and starch and which method includes, among other things, steeping said corn kernels and separating said steeped kernels from a steep liquor.

Abstract: Methods and compositions utilizing organism consisting of the strains within the order Clostridiales for improving health of animals. Disclosed methods comprise administering an effective dose of a composition comprising organism consisting of the strains within the order Clostridiales to an animal, including for example, a broiler or hen, thereby improving said animal's health.

Abstract: Single cell protein products and an integrated method for the production of ethanol and single cell protein. A single-cell protein product comprising on dry basis between 62% by weight and 75% by weight crude protein, at least 0.2% by weight butyric acid and at least three of, at least four of or all five of (i) between 5.5% by weight and 6.9% by weight lysine; (ii) between 2.1% by weight and 3.5% by weight methionine; (iii) between 3.1% by weight and 4.4% by weight threonine; (iv) between 2.0% by weight and 3.3% by weight tryptophan and (v) between 2.5% by weight and 4.5% by weight crude fat.

Abstract: A proteinic biomass preparation comprising a non-native organism of the Clostridia class, which organism expresses (i) a modified aspartate kinase; (ii) a modified homoserine dehydrogenase; (iii) a modified homoserine kinase; (iv) a modified anthranilate synthase; (v) a functional lycopene pathway and the genes crtY, crtW, and crtZ; and/or (vi) a functional oleic acid pathway and the four gene operon (pfaABCD). Methods of producing proteinic biomass preparations are also described.

Abstract: The present invention provides microorganisms capable of converting acetyl-coA into crotyl alcohol as well as fermentation methods for producing crotyl alcohol, either alone, or in combination with acetone and/or isopropanol. The microorganisms may be genetically engineered to express and/or disrupt one or more of the following enzymes: acetaldehyde dehydrogenase, alcohol dehydrogenase, bifunctional acetaldehyde/alcohol dehydrogenase, aldehyde oxidoreductase, phosphotransacetylase, acetate kinase, CoA-transferase A, CoA-transferase B, acetoacetate decarboxylase, secondary alcohol dehydrogenase, butyryl-CoA dehydro genase (BCD), and/or trans-2-enoyl-CoA reductase (TER).

Abstract: High cell density anaerobic fermentation for protein expression. A method for producing a protein is described, including providing a modified Butyribacterium methylotrophicum (Bme) strain host; providing a fermentation medium including a carbon source and a nitrogen source; culturing the modified host in the fermentation medium under anaerobic conditions, where the carbon source is metabolized and a fermentation broth including at least one protein is formed and optionally separating the protein; where the Bme cell density in the fermentation broth is greater than 15 g/L.

Abstract: An integrated wet-mill method for the production of ethanol and single cell protein. An integrated method for the production of ethanol and a single cell protein product is described including providing corn kernels, which kernels comprise germ, fiber, protein and starch and which method includes, among other things, steeping said corn kernels and separating said steeped kernels from a steep liquor.

Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.