Abstract: Computing systems, computer-readable media and methods are disclosed. In the computing system, an image capture device captures an image of a protein array including spots of predetermined proteins, wherein some of the spots have bound to a biological material having individual-specific antibodies to form immune complexes and some of the immune complexes have interacted with a detection agent to generate a visible image therefrom. The computing system may be operably coupled to the image capture device and processes the captured image of the protein array to determine control locations of a plurality of control spots in known locations on the protein array. The computing system also extrapolates expected locations of all other spots on the protein array from the control locations.

Abstract: A sample of a biological material having individual-specific antibodies is contacted with an array of less than about 200 proteins on a support to bind some of the individual-specific antibodies to the proteins of the array to form immune complexes. A detection agent with an interacting protein for conjugation to a marker is applied to the array to detect the immune complexes and obtain an antibody profile, which is compared to a known antibody profile obtained from an individual. The array may further include control spots including human IgG to form control complexes and volume assessment spots including volume determination proteins to form volume complexes. Intensity of the control complexes may be detected to determine if results of the identifying are complete. Intensity of the volume complexes may be detected to determine if a volume of the sample is sufficient for an accurate result.