Abstract: The present disclosure discloses a medium for primary isolation of Porphyromonas gingivalis, and a method for preparing the medium and use thereof. The medium for primary isolation of Porphyromonas gingivalis includes the following components in parts by weight: 10-20 parts of a mixed peptone, 5-10 parts of a yeast extract powder, 1-5 parts of sodium chloride, 10-15 parts of agar, 0.5-1.0 parts of glucose, 0.2-0.8 parts of sodium bicarbonate, 0.1-0.5 parts of an L-cysteine salt, 0.1-0.5 parts of soluble sodium pyrophosphate, 0.0001-0.0005 parts of hemin, 0.00001-0.00005 parts of vitamin K, 500-1000 parts of water and 5-10 parts of a sterile defiberized sheep blood. By adopting the medium for primary isolation of Porphyromonas gingivalis provided by examples of the present disclosure, a culture period of primary isolation of Porphyromonas gingivalis can be greatly shortened, and the primary isolation of Porphyromonas gingivalis can be quickly conducted.