Patents by Inventor Sheila Macintyre-Ayane

Sheila Macintyre-Ayane has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Microbial protein expression system
    Patent number: 6919198
    Abstract: The present invention provides bacterial strains for secretion of soluble biologically active recombinant heterologous proteins into periplasm or on a surface/into a cultivation medium of bacteria. The invention exploits the secretion system of Gram-negative bacteria including periplasmic chaperones and usher/secretin proteins. For accomplishing the aim, the bacterial strains simultaneously express the fusion protein (signal peptide)-(mature hecterologous protein)-(subunit of a bacterial surface structure, Caf1), periplasmic chaperone specific for the subunit, and outer membrane usher/secretin protein specific for the subunit. Secretion of fusion proteins: (signal peptide of Caf1)-(mature human IL-1?)-(mature Caf1), (signal peptide of Caf1)-(mature human GM-CSF)-(mature Caf1), and (signal peptide of Caf1)-(mature human IL-1ra)-(mature Caf1) that were expressed in Escherichia coli simultaneously with the periplasmic chaperone Caf1M and the usher/secretin protein Caf1A are examples of the use of the invention.
    Type: Grant
    Filed: May 3, 2000
    Date of Patent: July 19, 2005
    Assignee: Biotecnol S.A.
    Inventors: Timo Korpela, Sheila Macintyre-Ayane, Anton Zavialov, Natalia Battchikova, Lada Petrovskaya, Vladimir Zav'yalov, Galina Petrovna Korobko, Vyacheslav Korobko