Abstract: The present disclosure generally relates to digital microfluidic devices and associated methods. Some digital microfluidic devices described herein may be particularly suitable for manipulating droplets suitable for hosting cell growth. For instance, some digital microfluidic devices may include one or more features that assists with measuring and/or adjusting a property of one or more droplets during cell growth. As another example, some digital microfluidic devices may include one or more features that assist with performing a time-series measurement of one or more properties of a population of cells growing in a droplet. Such digital microfluidic devices may advantageously allow cell growth conditions to be recorded and/or adjusted during cell growth, which may enhance understanding of how various parameters affect cell growth and/or control of cell growth based on such knowledge.