Patents by Inventor Shelley Nelson

Shelley Nelson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Surface of a vessel with polystyrene, nitrogen, oxygen and a static sessile contact angle for attachment and cultivation of cells
    Patent number: 11001802
    Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: May 11, 2021
    Assignee: Nunc A/S
    Inventors: Benjamin Fryer, Shelley Nelson, Villy Nielsen, Tina Kristensen Marwood, Thomas Brevig
  • METHODS, SURFACE MODIFIED PLATES AND COMPOSITIONS FOR CELL ATTACHMENT, CULTIVATION AND DETACHMENT
    Publication number: 20190249138
    Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
    Type: Application
    Filed: August 31, 2018
    Publication date: August 15, 2019
    Applicants: Janssen Biotech, Inc., Nunc A/S
    Inventors: Benjamin Fryer, Shelley Nelson, Villy Nielsen, Tina Kristensen Marwood, Thomas Brevig
  • Methods for suspension cultures of human pluripotent stem cells
    Patent number: 10377989
    Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation.
    Type: Grant
    Filed: December 30, 2013
    Date of Patent: August 13, 2019
    Assignee: Janssen Biotech, Inc.
    Inventors: Benjamin Fryer, Daina Laniauskas, Marcia Blackmoore, Haiyun Wang, Kostadinka LiLova, Shelley Nelson, Elizabeth Rosocha
  • Method for making human pluripotent suspension cultures and cells derived therefrom
    Patent number: 10370644
    Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.
    Type: Grant
    Filed: June 17, 2014
    Date of Patent: August 6, 2019
    Assignee: Janssen Biotech, Inc.
    Inventors: Benjamin Fryer, Daina Laniauskas, Marcia Blackmoore, Haiyun Wang, Kostadinka Lilova, Shelley Nelson, Elizabeth Rosocha
  • Methods for producing single pluripotent stem cells and differentiation thereof
    Patent number: 10316293
    Abstract: The present invention relates to the field of pluripotent stem cell culture and methods facilitate pluripotent stem cell culture at industrial levels.
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: June 11, 2019
    Assignee: Janssen Biotech, Inc.
    Inventor: Shelley Nelson
  • PLURIPOTENT STEM CELL CULTURE ON MICRO-CARRIERS
    Publication number: 20180265842
    Abstract: The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on micro-carriers.
    Type: Application
    Filed: May 14, 2018
    Publication date: September 20, 2018
    Applicant: JANSSEN BIOTECH, INC.
    Inventor: Shelley Nelson
  • Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
    Patent number: 10066203
    Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
    Type: Grant
    Filed: February 19, 2009
    Date of Patent: September 4, 2018
    Assignees: Janssen Biotech Inc., Nunc A/S
    Inventors: Benjamin Fryer, Shelley Nelson, Villy Nielsen, Tina Kristensen Marwood, Thomas Brevig
  • Pluripotent stem cell culture on micro-carriers
    Patent number: 9969972
    Abstract: The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on micro-carriers.
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: May 15, 2018
    Assignee: Janssen Biotech, Inc.
    Inventor: Shelley Nelson
  • Single Pluripotent Stem Cell Culture
    Publication number: 20150275179
    Abstract: The present invention relates to the field of pluripotent stem cell culture and methods facilitate pluripotent stem cell culture at industrial levels.
    Type: Application
    Filed: June 10, 2015
    Publication date: October 1, 2015
    Applicant: LifeScan, Inc.
    Inventor: Shelley Nelson
  • Single pluripotent stem cell culture
    Patent number: 9080145
    Abstract: The present invention relates to the field of pluripotent stem cell culture and methods facilitate pluripotent stem cell culture at industrial levels.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: July 14, 2015
    Assignee: LifeScan Corporation
    Inventor: Shelley Nelson
  • Pluripotent Stem Cell Culture on Micro-Carriers
    Publication number: 20150166950
    Abstract: The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on micro-carriers.
    Type: Application
    Filed: February 25, 2015
    Publication date: June 18, 2015
    Applicant: JANSSEN BIOTECH, INC.
    Inventor: Shelley Nelson
  • SUSPENSION AND CLUSTERING OF HUMAN PLURIPOTENT STEM CELLS FOR DIFFERENTIATION INTO PANCREATIC ENDOCRINE CELLS
    Publication number: 20140295552
    Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.
    Type: Application
    Filed: June 17, 2014
    Publication date: October 2, 2014
    Inventors: Benjamin FRYER, Daina Laniauskas, Marcia Blackmoore, Haiyun Wang, Kostadinka Lilova, Shelley Nelson, Elizabeth Rosocha
  • Pluripotent Stem Cell Culture on Micro-Carriers
    Publication number: 20100124781
    Abstract: The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on micro-carriers.
    Type: Application
    Filed: November 19, 2009
    Publication date: May 20, 2010
    Inventor: Shelley Nelson
  • SINGLE PLURIPOTENT STEM CELL CULTURE
    Publication number: 20090325294
    Abstract: The present invention relates to the field of pluripotent stem cell culture and methods facilitate pluripotent stem cell culture at industrial levels.
    Type: Application
    Filed: June 30, 2008
    Publication date: December 31, 2009
    Inventor: Shelley Nelson
  • METHODS, SURFACE MODIFIED PLATES AND COMPOSITIONS FOR CELL ATTACHMENT, CULTIVATION AND DETACHMENT
    Publication number: 20090215177
    Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
    Type: Application
    Filed: February 19, 2009
    Publication date: August 27, 2009
    Inventors: Benjamin Fryer, Shelley Nelson, Villy Nielsen, Tina Kristensen Marwood, Thomas Brevig