Abstract: A detection method and a detection system for detecting objects of interest attached to a surface of a plurality of reporters, wherein the plurality of reporters are flowing in a microfluidic chip and illuminated by a light source. The detection method has following steps: obtaining a plurality of local surface plasmon resonance (LSPR) spectral images of each the plurality of the reporters individually, wherein each of the LSPR spectral images has a brightness of a long wavelength band (BA) and a brightness of a short wavelength band (BB); calculating a spectral image brightness contrast ? for each of the LSPR spectral images, wherein ? = B A - B B B A + B B ; and, defining a positive threshold for |?|?0.1.

Abstract: According to an embodiment of the present disclosure, an analysis apparatus may include a movable carrier, a sample providing device and a first analysis device. The movable carrier has at least one sample carry region, and moves the sample carry region to at least one collection position and an analysis position. The sample providing device provides a plurality of samples, wherein the sample carry region receives a portion of the samples at the collection position. The first analysis device may be aligned to the analysis position, and analyzes the samples on the sample carry region located at the analysis position.