Abstract: A skin nourishing ball is disclosed. The skin nourishing ball has as a transparent ball body that includes a casing made of fish-scale collagen and a fish embryo extract particle enclosed in the casing. The fish embryo extract is taken from a fertilized fish embryo and therefore highly stable against deterioration. In addition, the fish embryo extract is effective in reducing fine lines on the skin, improving skin elasticity, and enhancing the spontaneous moisture-retaining ability of skin. Due to the granular configuration of the skin nourishing ball, it is possible not only to take a specific quantity of such balls for use, but also to control the quantity with ease, thereby preventing the user from applying an excessive or insufficient quantity of the skin nourishing balls to the skin.

Abstract: A vector for expression of hEGF that is made by constructing a gene sequence of SEQ NO: 1 on an expression vector for the transformation of E. coli with said gene sequence to produce hEGF. Through said vector, high purity hEGF can be produced in E. coli and directly excreted from the cell, which means hEGF can be isolated and purified from E. coli culture. In other words, said vector for expression of hEGF can effectively simplify the production and purification procedures of hEGF and enhance its production.