Abstract: The present invention discloses a guiding element and a guiding assembly for spinal drilling operation. The guiding element is configured to be dispose at a vertebra which comprises a lamina. The guiding element comprises a main body, two connection parts, and two locating parts. The main body has two stand portions and each of the stand portion comprises a clamping part. Each of the connection parts comprises a first end and a second end. The two first ends extends away from each other. Each locating part connects to each stand portion through each connection part. Each clamping part comprises two hook portions. Each side, which is configured to be in contact against the vertebra, of the hook portions has a curvature corresponding to the surface of the lamina, so that the main body is configured to be disposed at the lamina and across the vertebra through the hook portions.