Abstract: A detection system for the interaction between known molecules and proteins based on covalent connection and an identification or verification method thereof are disclosed. The detection system comprises: a) streptavidin-short peptide tetramer; b) PafA enzyme; and c) Biotin-modified known molecules. After a known molecule interacts with a protein, streptavidin-short peptide tetramer can efficiently capture interacting proteins of known molecules under mild conditions. Then, under the catalysis of PafA enzyme, the interaction between short peptides and known molecules is made into protein covalent binding, so that the non-covalent binding between known molecules and proteins is converted into covalent binding between streptavidin and protein, and then analysis, separation and identification are carried out.

Abstract: A high-throughput and rapid nucleic acids detection method based on capillary microarrays comprises the steps that firstly, microarray containing a number of hydrophilic and vertical micro-channels is fabricated by capillary assembling, casting and machining, and the outer surface of the capillary array is coated with super-hydrophobic Ultra-Ever Dry paint; secondly, different primer sets are individually loaded into the micro-channels and air-dried to adhere them on the inner surface, and then the microarray is anchored into a reaction tube; thirdly, the reaction mixture is introduced into every microchannel at once through capillary force by a special designed sample-loading adaptor, and then the amplification reaction is performed in the temperature control device; and finally, the fluorescence can either be measured continually during the reaction for real-time detection or be recorded once in the end for endpoint detection. Moreover, the products can also be recovered for other use later.

Abstract: An apparatus for dispersing microbes in a biological sample such as a liquid suspension sample and measuring turbidity of the liquid suspension is disclosed. The apparatus is configured for dispersing the microbes in a liquid suspension without destroying the integrity of the microbes and measure the turbidity of the sample suspension at the same time or in the same sample set up without having to move the microbe sample from one equipment to another.

Abstract: The present invention discloses a high-throughput and rapid nucleic acids detection method based on capillary microarrays, and belongs to the technical field of biomedicine.

Abstract: This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

Abstract: This invention relates generally to the field of analyte assays. In particular, the invention provides a device for analyzing an analyte, which device comprises, inter alia, various means for moving analytes and other items to facilitate binding between analytes and their binding reagents immobilized on a surface and to facilitate clearance of undesirable items away from analyte-binding reagent interaction area to reduce background noise in the assay. Methods for analyzing an analyte using the devices are also disclosed.

Abstract: An apparatus for dispersing microbes in a biological sample such as a liquid suspension sample and measuring turbidity of the liquid suspension is disclosed. The apparatus is configured for dispersing the microbes in a liquid suspension without destroying the integrity of the microbes and measure the turbidity of the sample suspension at the same time or in the same sample set up without having to move the microbe sample from one equipment to another.

Abstract: This invention relates generally to the field of analyte assays. In particular, the invention provides a device for analyzing an analyte, which device comprises, inter alia, various means for moving analytes and other items to facilitate binding between analytes and their binding reagents immobilized on a surface and to facilitate clearance of undesirable items away from analyte-binding reagent interaction area to reduce background noise in the assay. Methods for analyzing an analyte using the devices are also disclosed.

Abstract: This invention relates generally to the field of PCR. In particular, the invention provides methods, compositions and kits for optimizing multiplex PCR primers using, inter alia, a plurality of 5? and 3? specific primers and a 5? and a 3? universal primer at various ratios.

Abstract: This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

Abstract: This invention relates generally to the field of virus detection. In particular, the invention provides chips, probes, primers, kits and methods for amplifying and detecting SARS-CoV nucleotide sequence. The clinical and other uses of the present chips, probes, primers, kits and methods are also contemplated.