Abstract: The present disclosure relates to systems and methods for analysis of proteins, more in particular to nanopore systems, devices and methods for single-molecule protein analysis and sequencing. Provided is a method for translocating a target protein through a nanopore, the nanopore being comprised in a membrane separating a fluidic chamber of a nanopore system into a cis side and a trans side, comprising: (a) allowing a protein translocase in solution to capture and form a complex with the target protein to be translocated; (b) contacting the translocase-target protein complex with the cis side of the nanopore and allowing for translocation of the target protein to the trans side; wherein the nanopore system has a cis to trans electro-osmotic force (EOF) resulting from a large net ionic current flow cis-to-trans relative to the total ionic current flow, so that the target protein is captured in the nanopore with on top of the nanopore the translocase controlling the translocation.

Abstract: The invention relates to the field of nanopores and the use thereof in analyzing biopolymers, including polypeptides and polynucleotides. Provided is an artificial nanopore comprising a multimeric assembly of subunits, each subunit comprising (i) the transmembrane (TM) sequence of a ?-barrel or ?-helical pore forming protein fused to the amino acid sequence of (ii) a subunit of a ring-forming protein capable of controlling the transport of a polypeptide or polynucleotide across the TM region of the assembly.

Abstract: The present disclosure relates to systems and methods for analysis of proteins, more in particular to nanopore systems, devices and methods for single-molecule protein analysis and sequencing. Provided is a method for translocating a target protein through a nanopore, the nanopore being comprised in a membrane separating a fluidic chamber of a nanopore system into a cis side and a trans side, comprising: (a) allowing a protein translocase in solution to capture and form a complex with the target protein to be translocated; (b) contacting the translocase-target protein complex with the cis side of the nanopore and allowing for translocation of the target protein to the trans side; wherein the nanopore system has a cis to trans electro-osmotic force (EOF) resulting from a large net ionic current flow cis-to-trans relative to the total ionic current flow, so that the target protein is captured in the nanopore with on top of the nanopore the translocase controlling the translocation.

Abstract: An optoelectronic apparatus (200) and an optoelectronic integration method are disclosed, so that bandwidth for signal transmission can be improved, and signal transmission performance is improved. The optoelectronic apparatus (200) includes: a printed circuit board PCB (201), where a first substrate (203) and a second substrate (205) are separately disposed on the PCB (201), an application specific integrated circuit ASIC (202) is disposed on the first substrate (203), and an optoelectronic component (204) is disposed on the second substrate (205); and a flexible printed circuit FPC (206), where a first end of the FPC (206) is disposed on an upper surface of the first substrate (203) and is electrically connected to the ASIC (202), and a second end of the FPC (206) is disposed on the second substrate (205) and is electrically connected to the optoelectronic component (204).

Abstract: The invention relates to the field of nanopores and the use thereof in analyzing biopolymers, including polypeptides and polynucleotides. Provided is an artificial nanopore comprising a multimeric assembly of subunits, each subunit comprising (i) the transmembrane (TM) sequence of a ?-barrel or ?-helical pore forming protein fused to the amino acid sequence of (ii) a subunit of a ring-forming protein capable of controlling the transport of a polypeptide or polynucleotide across the TM region of the assembly.

Abstract: The invention provides a method and apparatus for managing end nodes of a Low-Power Wide Area Network. A mode change command indicating a desire to switch the operating mode of each of a plurality of end nodes is broadcast. A determination is made as to whether all the end nodes have switched mode to match the desired operating mode, and the mode change command is rebroadcast if it is determined that not all end nodes have appropriately switched mode.

Abstract: A receiver, method, terminal device, visible light transmissive structure and system for decoding light signals and light signals received in an area of overlap of light rays emanating from different lighting devices, each transmitting unique ID data. Light rays (45, 46, 47) entering the receiver (39) are optically spread apart in longitudinal direction (x) of an array of photodetectors (21). The thus spread light rays (45a, 46a, 47a) are optically diverged and spread further over the radiant active surface (29) of a particular photodetector (22-28), thereby optimizing the amount of light received at a respective photodetector (22-28).

Abstract: A pipe body, wherein the pipe body (10) has a welded portion (11) at both ends of the pipe body, aligned in a widthwise direction of the pipe body (10). The thickness of the welded portion (11) decreases gradually from the inside to the outside of the pipe, and an outer end of the welded portion (11) is located at a center portion of the pipe body (10) in a thickness direction. The configuration of the welding structure enhances the strength of the welded portion of the pipe, so that the pipe will not crack easily when it is reworked by flaring or bending, thus having high reworkability. In addition, a pipe (100) made of the pipe body (10) and a method of making the pipe (100) are disclosed.

Abstract: This invention generally relates to commissioning in Zigbee Light Link (ZLL) networks. The proposed solution takes advantage of the existing Touchlink procedure in order to enable a controller device joining a ZLL network without having to Touchlink with every single lighting devices comprised in the ZLL network. This way, the one or more lighting devices of the ZLL network may be directly controlled by the newly joined controller device after a single Touchlink thanks to the introduction of a commissioning device. The commissioning device may be configured to provide specific joining information and binding information to the controller device. Further, a timer in the commissioning device may be triggered to lapse after a given period of time where the controller may be disconnected from the ZLL network.

Abstract: The invention provides a method and apparatus for managing end nodes of a Low-Power Wide Area Network. A mode change command indicating a desire to switch the operating mode of each of a plurality of end nodes is broadcast. A determination is made as to whether all the end nodes have switched mode to match the desired operating mode, and the mode change command is rebroadcast if it is determined that not all end nodes have appropriately switched mode.

Abstract: The present invention relates to a method and an apparatus for failure recovery in a network system, wherein a linked list is created, where each network node knows the network address of its neighbor node in at least one direction, wherein each network node sends a beacon to its neighbor node and has its neighbor node acknowledge receipt of the beacon, and wherein a network node checks with a switch device the port status of a port to which a neighbor node that has failed is connected in order to identify a replacement node.

Abstract: A receiver, method, terminal device, visible light transmissive structure and system for decoding light signals and light signals received in an area of overlap of light rays emanating from different lighting devices, each transmitting unique ID data. Light rays (45, 46, 47) entering the receiver (39) are optically spread apart in longitudinal direction (x) of an array of photodetectors (21). The thus spread light rays (45a, 46a, 47a) are optically diverged and spread further over the radiant active surface (29) of a particular photodetector (22-28), thereby optimizing the amount of light received at a respective photodetector (22-28).

Abstract: The disclosure provides a digital video content security authentication method, wherein the method comprises: a secret information embedding step of selectively modifying a pixel grayscale value for each frame image in a digital video so as to complete the embedding of 1-bit secret information; and a secret information extraction and video authentication step of carrying out secret information extraction from the digital video in which the secret information is embedded according to a key so as to realize the security authentication of the digital video content. The disclosure also provides a digital video content security authentication system. According to the technical scheme provided by the disclosure, the authentication security is improved on the basis of not influencing user experience.

Abstract: A pipe body, wherein the pipe body (10) has a welded portion (11) at both ends of the pipe body, aligned in a widthwise direction of the pipe body (10). The thickness of the welded portion (11) decreases gradually from the inside to the outside of the pipe, and an outer end of the welded portion (11) is located at a center portion of the pipe body (10) in a thickness direction. The configuration of the welding structure enhances the strength of the welded portion of the pipe, so that the pipe will not crack easily when it is reworked by flaring or bending, thus having high reworkability. In addition, a pipe (100) made of the pipe body (10) and a method of making the pipe (100) are disclosed.

Abstract: The disclosure provides a digital video content security authentication method, wherein the method comprises: a secret information embedding step of selectively modifying a pixel grayscale value for each frame image in a digital video so as to complete the embedding of 1-bit secret information; and a secret information extraction and video authentication step of carrying out secret information extraction from the digital video in which the secret information is embedded according to a key so as to realize the security authentication of the digital video content. The disclosure also provides a digital video content security authentication system. According to the technical scheme provided by the disclosure, the authentication security is improved on the basis of not influencing user experience.

Abstract: The present invention relates to a method and an apparatus for failure recovery in a network system, wherein a linked list is created, where each network node knows the network address of its neighbor node in at least one direction, wherein each network node sends a beacon to its neighbor node and has its neighbor node acknowledge receipt of the beacon, and wherein a network node checks with a switch device the port status of a port to which a neighbor node that has failed is connected in order to identify a replacement node.

Abstract: This invention generally relates to commissioning in Zigbee Light Link (ZLL) networks. The proposed solution takes advantage of the existing Touchlink procedure in order to enable a controller device joining a ZLL network without having to Touchlink with every single lighting devices comprised in the ZLL network. This way, the one or more lighting devices of the ZLL network may be directly controlled by the newly joined controller device after a single Touchlink thanks to the introduction of a commissioning device. The commissioning device may be configured to provide specific joining information and binding information to the controller device. Further, a timer in the commissioning device may be triggered to lapse after a given period of time where the controller may be disconnected from the ZLL network.

Abstract: An online game anti-cheating method is provided. The method includes sending cheating program characteristics to a client and receiving characteristics matching with cheating program characteristics reported by the client. The method also includes matching the reported characteristics with characteristics in at least one stored characteristic list and obtaining a determination result based on a matching result. Further, the method includes returning the determination result to the client and to cause the client to react to the determination result.

Abstract: An online game anti-cheating method is provided. The method includes sending cheating program characteristics to a client and receiving characteristics matching with cheating program characteristics reported by the client. The method also includes matching the reported characteristics with characteristics in at least one stored characteristic list and obtaining a determination result based on a matching result. Further, the method includes returning the determination result to the client and to cause the client to react to the determination result.